Studies On Techniques For Secondary Metabolites Culture In Vitro Of Aloe

Aloe, species containing aloin using in medicine for diminishing inflammation, cosmetics and sanitarian, employed as the materials producing the secondary metabolites in vitro. This thesis is of techniques for the tissue culture and callus production using A.arboresens Mill. As the results, dropped the aloe explants into the liquids containing the sodium hypochlorite 1.5% for 15min(explant is shorter than 2cm) and 2% for 10min(explant is longer than 2cm), decontaminated 84% and 95%, respectively. BAP is the mostly important factor in the multiplication. Rooting is stimulated more effectively with IBA than NAA. The plants transplanted for 5months survived 90%. Calli occurred 92.48%from the explants of the stem or apexes with 2cm long on the medium of ER + NAA15mg/L + 2,4-D4.0mg/L + Sugar30g/L. The callus grow better if added Vc 5mg/L and AgNO₃ l0mg/L in the medium. In suspension culture, the cell in dark grow fast in the medium of ER +NAA2.0mg/L+KT0.02mg/L+Sugar45g/L, regulated pH at 5.8. The growth trend of calli and suspension cells is recorded in a logistic growth curves(S type), respectively. The callus maximum growth yielded 20.8g/LDW each 25 day culture. The anthraquinone production high yielded at the lag and stationary states of the growth curves, respectively. They were weighted 0.134mg/g FW in calli and 0.201mg/g FW in suspension cells, respectively. The allocations of the secondary metabolites in leaf show histochemically in the chlorenchyma aloin cells mainly, whilst find the yellow analog materials in the cell gaps,could not find this crstal in the aquiferous. In calli ,found the yellow analog materials in the big parenchymatous cells.