Isolation And Identification Of Bacteria In The Nodule Structure From Podocarpus Brevifolius And Podocarpus Nagi

23 bacterium endophyte were isolated from the root nodule of Podocarpus brevifolius (Stapf) Foxw. and Podocarpus nagi from six different places in the campus of Guangxi University and research institute of forestry of Guangxi. They are pole form fungus, gram-negative, and they have flagellums. According to spore, all strains can be divided into two bacterium type groups: 13 bacterium do not have spore and 10 have spore. Based on Bergey's Manual of Determinative Bacteriology, one herd of bacteria to be appraised: 13 bacterium producing spore were identified through experiment of systemic identification, combining they made Locust nodulation after inoculating and made various changes of locust roots and made original host nodulation. Among them, two strains (C4 and C4Y41 ) belong to Rhizobium finally; Based on phenotypic identification and 16S rDNA sequence analysis, 10 bacterium with spore were identified as Bacillus subtilis. At the same time we found that 10 strains of Bacillus subtilis made Locust nodulation after inoculating and made original host nodulation. Finally, 50% strains can make Locust increased weight with degree of 0.05%-37.97%, thus we can know, some strains have certainfacilitation to the growth of plant. With specific primer designed, PCR product nifA gene fragment was amplified by PCR. The fragment was sequenced and closely related to Klebsiella pneumoniae nif gene cluster with 99% overall similarity.