Study On The Biological Effect Of Ultrasound In Nicotiana Tabacum L.

Abstract: Tabacco(Nicotiana tabacum L.)origined from South America, planted in temperate zone and tropical belt in the world. The leaf is the material of the cigarette, the plant also can be used as agriculture helminthic, and it's one of the important economic crops in our country. In the long research, tobacco became the preferred material in botany experiment, because of strong propagation ability, strong antivirus ability in tissue culture; some important meaning reseachment results have achieved. The effect of envirmental stresses on the growth and development of plant cells and tissues is one of important fields in plant physiology. In the experiment, we ultilized it to develop ultrasound biology function reseach, on the base of different explant reproduction system and cell suspension culture system establishment, we went on the reseachment in growth, physiology, cell ultrastructure, provided theory support for ultrasound biology effect.1. The experiment went on the reproduction system researchment of NC89 tobacco plantlet leaf and roots. Respectively ensured the best medium of adventitious bud induced by leaf was 2/3MS+BA0.5mg/l+NAA0.1mg/l, the best medium of adventitious root induced by adventitious bud was 2/3MS+BA1.0mg/l+NAA1.0mg/l, the best medium of adventitious bud induced by root was MS+BA0.3mg/l+ IBA0.1mg/1, the best medium of adventitious root induced by adventitious bud was MS+BA0.1mg/l+NAA1.5mg/l. It convinced that the higher concentrations rate of auxins and cytokinins was benefit for the adventitious buds differentiation, on the contrary, the slower concentration of that was benefit for the adventitious roots differentiation.2. The acquisition of friable calli and the establishment of the cell suspension cultures of tobacco NC89 leaf were studied. Firstly, the results indicated that the 2/3MS medium, which contained BA0.5mg/l and NAA0.1mg/l was suitable for calli differentiating from the leaf;the 2/3MS medium,which contained BA0.5mg/l, NAA0.1mg/l, 3.33248mg/l Ca (NO₃)₂ was suitable for calli subculture, so the friable calli can be acquired rapidly and the cell suspension cultures had been successfully established,maybe it's related to the requirement quantity of the Ca⁺⁺ during these calli mushroom, besides some important biology function of the Ca⁺⁺. Secondly, the results indicated that the MS medium, which contained ZT 0.5mg/l and IAA 0.05mg/l was suitable for calli differentiating from the root; the MS medium, which contained KT0.5mg/l and IAA0.05mg/l was suitable for calli subculture.3. We study the effect of ultrasound on SOD, POD and CAT in cell suspension culture of Nicotiana tabacum L. The results showed that ultrasound could induct the activity of SOD evidently, and the effect of seven minute ultrasound was the most distinction. At the same time the different ultrasound period could make the activity peak of SOD and CAT retardate three days, but the ultrasound induction effect on POD and CAT were controlled and not clearly.We can draw a conclusion that addition of ultrasound to the cell suspension culture of Nicotiana tabacum L.could cause the cells to elicit the defence response and induct the activities of relative enzymes.Maybe...