SSR Analysis Of Genetic Diversity And Conservation Of Oryza Rufipogon Along Haojiang River In Guangxi Zhuang Autonomous Region

The patterns of genetic diversity and genetic structure of 12 natural populations of Oryza rufipogon alongside Haojiang River in China were investigated with 24 SSR markers. The results are as follows: Twenty-four microsatellites were polymorphic and were used to assess the genetic diversity of 343 individuals from 12 Oryza rufipogon natural populations alongside Haojiang River, Guangxi Zhuang Autonomous Region in China. Total 209 alleles were detected and showed high genetic diversity. The number of alleles per locus (A) was 8.58. The effective numbers of alleles (Ae) was 3.71. The average expected heterozygosity (He) and observed heterozygosity (Ho) were 0.62 and 0.32 respectively, while the Shannon-Weaver index (I) was 1.37. The genetic diversity varied among different populations of Oryza rufipogon from Haojiang River. The percentage of polymorphic loci of each population ranged from 79% at Population L to 100% at Population B, G and H with the mean of 92.36%. The mean number of alleles per locus (A) was 2.21~5.29 in each population. The effective number of alleles per locus (Ae) ranged from 1.78 at Population K and 2.88 at Population B. The Shnnon's information index (I) ranged from 0.55 at population L to 1.11 at population B. The mean number of heterozygosity (He) ranged between 0.33 at Population L and 0.56 at Population B. The value of Ho ranged from 0.24 at Population E and 0.40 at Population I. The highest genetic diversity was observed in Population B from the downstream, the moderate genetic diversity was found in Population I and J from the middle and the lowest genetic diversity was found in Population K and L from the upstream. So the pattern of genetic diversity of wild rice alongside the river was obtained. Some rare alleles which existed only in one population and whose frequency exceeded 1% were detected by SSR. Sixty-five of 209 alleles appeared only in one or two populations and 35 alleles appeared only in one population, among which 12 appeared only in Population B. High gene differentiation coefficient (Fst=0.30) and gene flow (Nm=0.58) were detected, indicated that 30.23% of the total genetic variation existed among populations. Hardy-Weinberg test infered that the Oryza rufipogon populations from Haojiang River...