Screening Synergists For Bacillus Cereus A-47 And Researching The Mechanisms Of Interaction

B .cereus A-47 strains of the beneficial endophytic bacteria screened from wheat in our laboratory, which can prevent plant disease and promote growing of wheat. As microcological preparation, it has been commercialized and been widely used on farming yields. However, nearly all of microcological preparations are often influenced by environment so that the efficiency appears unstable. And addition of some synergists to could solve this problem. In this paper, efficiency of some chemical substances was tested to enhance the action of B .cereus A-47 in wheat. The action mechanism of synergism wasalso studied.Through the first bolting and the second bolting, the results showed that many compounds were able to increase the efficiency of B.cereus for promoting the wheat to grow to varying degrees. Among these substances, N MF . CaCl₂ brown sugar and chitin showed synergism effect obviously.We tested the effect of synergism on the growth of B.cereus. The results indicated that all of the synergists can promote bacillus to propagate to different degree. We also tested the effec of synergism on the colonization of B .cereus A-47 in wheat and found that adding NF CaCl₂ N can enhance the colonization of B .cereus in wheat during seedling stage, while chitin and brown sugar have no synergism on it. With high performance liquid chromatography (HPLC) method, we tested the content of zeatin in B.cereus and found that the additive of brown sugar and CaCl₂ can increase the concentration of zeatin greatly.Screening synergists on B . cereus in wheat and study of its action mechanisms will provide with some scientific evidence for selection and use of synergism, and it will establish a good foundation for application and popularization of endophytic bacillus products.