Study On Effectively Transforming Of Alliin And Improving Anti-heat Stress Performance Of Garlic Compounds In Broiler Chickens

This thesis studied on the effect to the enzyme catalytic reaction of alliin's transforming to allicin by different monfactors (reacting time temperature . pH activator),and seeking the best selection with the method of orthogonal test on the basis of these factors. With the best selection,alliin was effectively transformed and Garlic Compounds(coded GC) which include kinds of bioactive substances were produced . GC were added to the diet of broiler chickens under heat stress, the effects on growth and endocrine metabolism immunity anti-oxidation ability were investigated. Chapter I Study on effectively transforming of alliin1 Different monfactors (reacting time temperature pH activator) which affect the enzyme catalytic reaction of alliin transforming to allicin were measured by new method.The result showed that: reacting time 1.51k temperature 30℃ pH6.7, [Fe~2+]15 mmol/L are respectively the best condition. 2 The result by orthogonal test showed the best selection is reacting time 120min temperature 30℃ pH6.4 [Fe2+]15 mmol/L. 3 After transforming of alliin in the best reacting conditions,allicin in garlic compounds was determined,the result was 1.59%, significantly higher than that of productions under no dealing with and best conditions in modern craft. Chapter II. Study on improving anti-heat stress performance of GarlicCompounds in broiler chickensThree-week-old AA broilers were randomly divided into 4 groups,all groups were reared under 33℃.The GC of 0,50, 150 250mg/kg were added to basal diet of broiler chickens.This test was from 21st day to 42nd day.The test studied the effects of different doses of GC on anti-heat stress performance in broilers. Series I Study on GC promoting growth metabolism in heat stress broilersThe results showed that,Every dosage GC can very significantly increase the body weight gain and feed intake of heat-stress broiler chickens (P<0.01) significantly decrease the feed/gain,the group of 50 mg/kg decreased significantly, the other two groups decreased very significantly;compared with the control gro-up,the levels of serum hormones, serum glucose and serum total cholesterol of treated groups are: serum adrenocorticotropic hormone (ACTH) level was decreased, groups of 150 250mg/kg decreased significantly(P<0.05); glucagon was decreased insignificantly ; Triiodothyronine( T3) , thyronine ( T4) level were increased , the group of 250mg/kg increased significantly;the concentration of serum glucose was decreased, groups of 150, 250mg/Kg decreased significantly (P<0.05); serum total cholesterol(TC) level was decreased, the group of 250 mg/Kg decreased significantly(P<0.05).But,GC has no significant effects on the dress percentage ,semi-evicerated carcass weight rate ,evicerated carcass weight rate; GC can decrease the percentage of abdominal fat, groups of 50, 250 mg/ kg decreased significantly(P<0.05).Series II : Study on GC improving immune function and antioxidation performance in heat-stress broilersThe result showed: different doses of GC were added to the diet under hot circumstance, compared with the control group, serum Newcastle Disease (ND) -Hemagglutination Inhibition (HI)titer of treated groups in 5 and 15 days after the second vaccination increased significantly(P<0.05 or P<0.01), and in all testing days,the apparent level of antibody titer of treated groups were higher than control group; spleen index and thymus index of 250mg/kg group increased significantly(P<0.05), immune function was improved ; the level of Malondialde-hyde (MDA) in serum on 42nd day decreased significantly(P<0.05), antioxidationability was improved.