Establishment DNA Fingerprinting And Evaluation Genetic Diversity Of Transgenic Insect-resistant Cotton

In this paper, 52 transgenic insect-resistant cotton varieties of Gossypium hirsutum from China and abroad, have been used to investigate fiber quality traits, construct cluster dengrogram. Based on the SSR and AFLP analysis, we analysised the diversity and constructed the fingerprinting of the cultivars. The main results were as follows:1. The analysis of the fiber quality traits showed that there were difference among the experiment materials. The results showed that the transgenic insect-resistant cotton can't meet the needs of textile industry enough. The fiber length varieties ranged from 28 mm to 30 mm, the fiber strength was within 23.0³1.6 cN.tex^-1, the micronaire was from 4.0⁵.6 and the lint percentage was from 3.6⁴.4. The variance of fiber strength was the biggest among the traits among the coefficients of variance.2. 13 polymophic primer combinations were selected to perform the SSR and AFLP fingerprinting. 32 cultivars have specific bands, accounted for 61.5%. Every primer combination was different in the identification efficiency of cultivars. No primer combination could distinguish all the cultivars.3. The analysis based the fiber quality traits showed that the Euclidean distance was among 0.56⁷.92 and the average Euclidean distance was 3.42. The pairwise Euclidean distance of the varieties ranged from 2.52 to 5.56. It showed that little difference existed among different tested varieties.4. The Euclidean distance was among 2.00⁵.57 and the average Euclidean distance was 4.21 by SSR and AFLP. The pairwise Euclidean distance of the varieties ranged from 3.73 to 4.75 which showed that the genetic basic among the tested varieties is not abundance.5. With the morphological characters, the materials all could be divided into three groups. With the two molecular, the materials all could be divided into three groups too. In every group, there were some cultivars from different breeding provinces. The cultivars in every group were not consistent in two methods.6. Six varieties(lines) were screened form 52 tested materials based on analysis of morphological characters and molecular markers. They were Zhongmiansuo30,...