Studies On High Frequency Somatic Embryogenesis And Regeneration Culture In Litchi

Litchi(Litchi chinesis Sonn) belongs to Sapindaceae family and or(?)g(?)m(?)ated in China and is a famous green fruit trees in south of China. It's difficult to culture (?)it(?)c(?)i in vitro, therefore, development of biotechnology in litchi is limited. In this rese(?)rch, anther of Feizhixiao, Shuidong, No.2 of Haigen and Sanshanjianyeli were used as materials t(?) study how to obtain embryogenic callus, to preserve and induce somatic embryo. And (?)hen (?)e(?)stablish and improve the high frequency somatic embryogenesis system and plant regenera(?)ion system of litchi. Expect to found the biotechnologic theory, technology and biotechnology breeding etc of litchi. The main research results are as follows:1. The embryogenic callus of several anthers of litchi was inducted, obtained embryogenic callus from Feizixiao, Shuidong and Sanshanjianyeli, and obtained no n-em(?)bryogenic callus from No.2 of Haigen. Found that the medium of MS+2,4-D2.0mg/L+NA A0.5(?)g/L+PVP500mg/L+ Sucrose50g/L adapted to induce callus of anther of litchi.2. The embryogenic callus of different species of litchi was comse(?)ved. Found that the medium of MS+mA3.0mg/L+BA1.0mg/L+Sucrose6%+Agar6.8% is the best to keep the embryogenic callus. Long-term preservation can be achieved by connb(?)ed the medium of MS+NAA3.0mg/L+BA1.0mg/L+ Sucrose6%+Agar6.8% and MS alter(?)atively. During the culture the liquid medium of MS should be used several times. Using the method, the embryogenic callus of Shuidong and Feizixiao was not affected. But the (?)o(?)-embryogenic callus of No.2 of Haigen was found easy to turn to embryogenic callus.3. The mediums that adapted to somatic induction of litchi emlbr(?genic callus were selected by using the orthogonal experiment design. In the medium of MS+BA0.5mg/L+ KT0.5mg/L+Sucrose6%+Agarl0mg/L+LH500mg/L+Honey400mg/L, so(?)atic embryos of Shuidong has been obtained while the somatic embryos of Feizixiao (?)eeded the medium of MS+KT0.5mg/L+NAA0.1mg/L+ Sucrose3%+ Agar6.8mg/L+Ac0.5%. The method that embryogenic callus cultured in liquid medium for two weeks and the n transferred to solid medium was best, produced a little of abnormality somatic embryos, and obtained identical...