Research On Improvement In Plant Regeneration In Wheat And Tansferring High Molecular Weight Glutenin Subunit 14 (HMW-GS14) Gene Into Wheat

High-molecular-weight glutenins which constitute of many subunits is a kind of important storage protein and occupy 10% of whole protein in seed. Plenty of studies showed that constitution of high-molecular-weight glutenin subunit (HMW-GS) play a signification role on Triticeae processing quality. It is an important subject that transfer gene into cultivate Triticeae to improve quality utilizing gene engineering. In this study, inducing rate diversity of these different genotypes under different differentiation and rooting mediums was compared. We can get two genotypes .Then with the gene gun method ,this experimnt transformed plasmid pBI-121 into the immature embryonic callus of the two wheat species at the different volumes of gold power, different distance to ,times of ,and using wink expression as well as regeneration and expression of gus to establish and optimize transformation system of gene gun method so as to use the system to transform wheat HMW-GS14 gene'section into wheat's immature callus and make it recombined. By PCR we have identified the regenerate wheat. The experiment indicate that (1)Genotype was not very important in wheat immature embryos callus induction, but it played an important role on the bud differentiation(bud formation rate differs much between callicoming from plants of different genotypes); (2) Popularly, to same genotypes the regeneration frequence of explant is that immature embryos exceed immature inflorescences (3) in this study, we also found that the optimum culture condition which different genotypes need is distinct; even to the same genotype the different explant need very different culture condition;(4)Proper pre-differentiation of callus before transferred to regeneration medium was found to improve the frequence of plant differentiation. Low temperature conduce to increase the frequnency of formation (5) It shows in this experiment,raising volumes of gold power can promote the positive rate of gus;but it's adverse to regeneration.That use 125μg/gun gold power and consider add times is benefit to improve transferring efficiency.(6) We have got three electropositive wheats though identified the regenerating wheats by PCR. According to experiment,we can conclude the gene have been transferred into these wheats. The transferring frequence is 0.183%.