The Clonging And Physiological Functional Analysis Of OsRLK1 In Rice (Oryza Sativa L.)

Receptor-like protein kinases (RLKs) are important mediators of signaling in Plant. RLKs contain an extracellular receptor domain (ectodomain), a single transmembrane domain and a cytoplasmic protein kinase domain which is modulate in response to a stimulus. A receptor protein kinase gene in rice designated OsRLKl was cloned. To investigate the the function of OsRLKl gene, we developed transgenic plants with reduction and overexpression of OsRLKl, and the fused gene of POsRLKr.GUS. OsRLKl is a transmembrane protein localized in cell membrane. It expressed in embryo, coleoptile, flower, primordia of crown roots and roots. OsRLKl was regulated by local nitrate supplement and exogenous cytokinin. But the RNAi and overexpressing transgenic plants showed the same response as wild-type plants to local nitrate treament or 0.1 μ M cytokinin treatment. The results indicate that as a member of multiple gene family, the function of OsRLKl may be redundant in rice.