The Expression Analysis Of Paralichthys Olivaceus Hepcidin Gene And The Antibacterial Effects Of Pagrus Major Recombinant Antimicrobial Peptide

Gene-encoded antimicrobial peptides are an ancient and pervasive component of the innate defense mechanisms They are found in diverse species and has the properties of low molecular mass, heat-resistance, broad-spectrum activity against pathogenic bacterial. Our reasearch mainly focuses on the expression analysis of Hepcidin gene during different embryonic developmental stages and the expression analysis of Hepcidin gene in several tissues. The antibacterial effects of recombinant antimicrobial peptide are also addressed.The samples of Paralichtys olivaceus embryos during developmental stages, which are 4h, 20h, 36h, 44h, 52h, 68h, 80h after fertilization, and the samples of liver, spleen, intestine, gill, heart, head kidney are collected. Using Trizol reagent RNA is extracred and cDNA is synthesised through reverse transcription. The result of RT-PCR assay of embryo-specific expression analysis of hepcidin transcript are that they are barely detected from 4h to36h, at low levers from 44h to 68h and at relatively high level at 80h. The result of RT-PCR assay of tissue-specific expression analysis of hepcidin transcript are that they are abundant in the liver, spleen and head kidney, to a lesser extent in the gill and heart, and can not be detected in intestine.The antibacterial effects of recombinant antimicrobial peptides on Escherichia coli (ATCC 25922), Edwardsiella tarda (As 1.187), Streptococcus mutans (As 1.2499), Candida albicans (As 2.2086), Staphylococcus aureus (As 1.2465), Aeromonas hydrophia (As 1.1801), Bacillus subtilis (As 1.2428), Pseudomonas aeruginosa (As 1.2387), Micrococcus lysoleikticus are performed. The result shows that recombinant antimicrobial peptides has antibacterial effects on Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (As 1.2387) and Aeromonas hydrophia (As 1.1801), while it has no effect on the others.