Detection Of Goose Parvovirus Using Indirect Immunohistochemistry And Indirect Immunofluorescence From Experimentally Infected In Paraffin-embedded Tissue And Distribting Regularity Of Goose Parvovirus In Infected Goslings

Indirect immunohistochemistry Staining(IIS) and Indirect immunofluorescence (â…¡F) for detecting Goose parvovirus were developed by anti-GPV IgG that came from rabbits which were inoculated by purified GPV. The two methods can detect strong positive signals in livers from which sacrificed GPV-mfected goslings, but without any positive signals in livers from negative control goslings and goslings infected with Riemerella antipestifer serotypel Escherichia coli (O₁,O₈), Salmonella. Pasteurella mitltocida, Gosling new type viral enteritis virus, respectively. We used the two methods to detect seventeen tissues and its results showed that GPV antigen located in nuclei mainly, in cytoplasm partly within cardiac muscle, liver, spleen, lung, kidney , cerebrum, thymusgland, pancreas, duodenum , blindgut, ileum .jejunum, rectum, bursa of Fabricius .We systematically investigated the consecutive developmental distribution regularity of goose parvovirus in artifically infected goslings respectively. The results of â…¡S indicated that there were some differences among the three groups (subcutaneously. orally, intranasally inoculated group). The results of subcutaneously inoculated group in different hours post inoculation: GPV antigen can be detected from kidney and liver at 8hpi; GPV antigen can be detedted from cardiac muscle, liver, spleen, kidney, duodenum, thymus at 12hpi; GPV antigen can be detected from ten tissues such as liver, spleen .kidney, and so on at 24hpi; GPV antigen can be detected from all tissues excepted for muscle, gullet and glandular stomach from 48 to 96 hpi; GPV antigen can't be detected from lung and pancreas at 192hpi, and the positive extent decreased gradually in other tissues: GPV antigen still can be detected from liver and duodenum at 576hpi. The results of infected orally group: the GPV antigen can be detected from liver at 8hpi: GPV antigen can be detected from cardiac muscle, liver, kidney, duodenum, bursa of Fabricius at 12hpi; GPV antigen can be detected from eight tissues such as lung, liver, thymus and so on at 24hpi: GPV antigen can be detected from twelve tissues such as liver.spleen,lung and so on at 48hpi; GPV antigen can be detected from all tissues excepted for muscle,gullet and glandular stomach from 96 to 144hpi; GPV antigen can not be detected from lung,pancreas and bursa of Fabricius at 192hpi, and the positive extent decreased gradually in others tissues; the GPV antigen still can be detected from liver and kidney at 576hpi. The result of intranasally infected group: GPV antigen can be detected from liver at 8hpi; GPV antigen can be detected from cardiac muscle, liver, kidney, spleen, duodenum, bursa of Fabricius at 12hpi; GPV antigen can be detected from eight tissues such as liver, spleen, kidney and so on at 24hpi; GPV antigen can be detected from twelve tissues such as liver,sp!een,lung and so on at 48hpi; GPV antigen can be detected from all tissues excepted for muscle, gullet and glandular stomach from 96 to 144 hpi; GPV antigen can not be detected from bursa of Fabricius at 192hpi; the GPV antigen still can be detected from liver and ileum at 576hpi.The result indicated that the positive rate of three inoculating methods is different in all collected tissues. The subsequence of positive rate in subcutaneously inoculated group: Iiver(17/22), kidney( 14/22), duodenum( 13/22). cardiac muscled2/22), jejunum(12/22), spleen (12/22), ileum(ll/22),cerebrum(10/22),thymus(9/22),cecum(8/22). rectum(6/22), bursa of Fabricius (6/22), lung(5/22),Pancreas(3/22). The subsequence of positive rate in orally inoculated group: liver( 18/22), kidney( 12/22), duodenum(12/22),cardiac muscle (12/22). jejunum(ll/22), spleen (10/22), ileum(10/22), thymus(9/22).cerebrum(7/22), cecum(6/22), lung(6/22),rectum(5/22), bursa of Fabricius (5/22), Pancreas(5/22). The subsequence of positive rate in intranasally inoculated group: Iiver( 17/22), kidney(l4/22), duodenum(ll/22),cardiac muscle( 11/22), jejunum( 11/22), spleen (11/22), ileum(10/22), thymus(10/22),cerebrum(7/22),bursa of Fabricius(7/22). cecum(6/22), lung(6/22), Pancreas (6/22), rectum(4/22).The results of IIF indicated that the distribution regularity of goose parvovirus in artifically infected gosling accorded with the results of IIS, although there were several differences in some individuality.The virus antigen can't be detected from any tissues of goslings at 4hpi and 50dpi by three routes of inoculation. The distribution rate of subcutaneous inoculation at 96hpi was faster than distribution rate of oral inoculation and intranasal inoculation at 144hpi. The max detection rate was the liver among the detected tissuses. During this research, we didn't detected the GPV antigen from glandular stomach, muscle and gullet using the two methods.