| The objective of this study was to improve the nutritive and feeding value of double-low rapeseed meal(DLRM) inclusion in diets for pigs and broilers. A feeding trial and slaughtering trial were conducted to determine the appropriate digestible Lysine level of DLRM-based diet for grower-finisher pigs, and to examine the effect of betaine on the improvement of available energy of the DLRM-based diets. The metabolism and growth trail with broilers was conducted to study both the optimum adding level of DLRM and favorable energy level of DLRM-based diets. The following are the results:1. The two factors system design was conducted to investigate the appropriate digestible Lysine level of DLRM-based diet for grower-finisher pigs and the appropriate antibiotics to control the potential toxic effect derived from glucosinolates. Based on the apparent ileal digestibility of lysine testing from Pengjian( 1999b), the experiment has arranged three available digestible Lysine levels (100%, 112% and 118% of the levels recommended by NRC, 1998 respectively, ), and the content of digestible lysine is 0.77%, 0.86%, 0.91% for growing phase and 0.61%, 0.68%, 0.72% for finishing phase. Each digestible lysine level was arranged with the antibiotics combinations A and B. The results indicated that during the growing phase(30~60kg) the level (0.77%) recommended by NRC (1998) can not meet the requirement of digestible lysine for the maximum growth potential. Better performance(ADG of 769~800g ) could be obtained by increase the digestible lysine level to 112%~118%(0.86%~0.91%) of diets.During finishing phase(60~ 100kg), however, with the increases in the digestible Lysine level, the growth performance of pigs decreased (827g vs 790g vs 750g), which has confirmed that during finishing period, the level (0.61%) recommended by NRC (1998) is appropriate. The two different antibiotics combinations A and B both can effectively control the diarrhea, and antibiotic B is better (P<0.05). The results confirmed that better growth performance and economic profit could be obtained by feeding double-low rapeseed meal-based diet formulated by digestible Lysine index, appropriate and appropriate bacteriophage additive could effecttively control the transformation of glucosinolate and the potential toxicity in vivo.2. a feeding and slaughtering trial were conducted to evaluate the effect of the betaine as an energy modifier. Digestible energy(DE) and lysine level were established based on the level recommended by NRC(1998) and the result of experiment l.Diet 1 based on corn-soybean meal was used as the positive control(DE is 13.9MJ/kg for 30kg~60kg and13.8MJ/kg for 60kg~100kg ), using different proportions of DLRM(10% and 12.5% for 30kg~60kg, 15.5% and 16% for 60~100kg) on an iso-nitrogen basis to replace the soybean meal in diet 1, formed the negative control 1 (deit 2) and 2(deit 4), whose digestible energy is decreased by 100kcal/kg and 150kcal/kg compared to the positive control, respectively. The two negative controls supplemented with 0.1% betaine formed diets 3 and 5. At the body weight of 100kg, 4 pigs(2 male and 2 female) from each diet were randomly selected to slaughter. The results indicated that no significant difference in backfat depth and fat:lean was observed among pigs on diets 2 , 4 and l(P>0.05), but pigs on diets 3 and 5 both had a decreased backfat depth in comparison to those on diet l(P<0.05). The effect of reduction in fat:lean was more obvious in diet whose energy was decreased 150kcal/kg than in diet whose energy was decreased 100kcal/kg(P=0.101). In a result, the betaine as an energy modifier was effective in the improvement of feeding value of double-low rapeseed's low available energy diet.3. The study established the appropriate adding level of double-low rapeseed meal in broiler diet. Using DLRM on an iso-nitrogen basis to replace soybean meal in the control(corn-soybean meal based diet), formed 5 diets, the replacement ratio of soybean meal was 0, 25%, 37.5%, 50%, 62.5% during weeks 0-3, and 0, 37.5% , 50%, 62.5%, 75% during weeks 4-6. the results showed that growth performance and economic profit of pigs on DLRM based diets than those on corn-soybean meal based diet. Higher weight gain(1902g vs 1844g, P<0.05) and more economic profit( 0.51 yuan more per )could be obtained in the replacement ratio of 37.5% during growing phase 1 (weeks 0 -3) and 50.0% in growing phase 2 (weeks 4-6). Even when the replacment proportion increases to 62.5% during growing phase 1 (weeks 0 -3), and to 75.0% during growing phase 2(weeks 4 -6), the growth and feeding efficiency were not effected(P>0.05).The above results indicated that DLRM is the excellent protein resource in the broiler diet.4. The study established the apropriate energy requirement of DLRM-based diet for broilers. Keeping the same replacement ratio of soybean meal by DLRM on an iso-nitrogen and the ratio of protein:energy in each trial groulp(the replacement ratio and protein:energy were 50% and 72.5g/Mcal for weeks 0-3, and 62.5% and 63.3 g/Mcal for weeks4~6 ). 4 trial diets were formulated to metabolizable energy 2.7Mkcal/kg, 2.8Mkcal/kg, 2.9Mkcal/kg , 3.0Mkcal/kg and crude protein 19.57%, 2.92%, 21.00%, 21.7% for weeks 0~3, and metabolizable energy 2.8Mkcal/kg, 2.9Mkcal/kg , 3.0Mkcal/kg, 3.1Mkcal/kg, and crude protein 17.72%, 18.35%, 19.00%, 19.62% for weeks 4-6. The results indicated that during phase 1 (weeks 0-3), the weight gain increases(430g vs 533g vs 542g vs 55 lg) with the increase of the diet density. While during phase 2 (weeks 4-6), In the energy level ranged from 2.8Mkcal/kg to 3.0Mkcal/kg, the weight gain(1107g vs 1426g vs 1446g) increases with the increase of the energy density. When the metabolisable energy was beyond 3.0Mkcal/kg , the weght gain decreased(1431g). Weighing the performance and economic profit, the most appropriatemetaboliazable energy level of double-low rapeseed-based diet for broiler is 2.8-3.0Mcal/kg for weeks 0-3, and 2.9-3.0Mcal/kg for weeks 4-6.
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