Genetic Diversity Analysis Of Mitochondrial DNA Sequences In G.maculatum

G. maculatum belonging to Glyptosternum, in family Sisoridae and order Siluriformes, is one of the Glyptosternoid catfish species. This species only distributed in the middle region of Yaluzangbujiang (Tsaopo) in Tibet, and in Brahmaputra in India. There are some reports about G maculatum. Most of them are about its morphology and bionomics, but few about its genetic diversity.In this study, we obtained the complete nucleotide sequences of the mitochondrial DNA cytochrome b and D-loop by PCR. We analyzed these sequences by using Clustal X, Seaview, Annhyb and Mega 2.1. The main results are:1. In the experiment, we sequenced twenty-seven nucleotide sequences of the complete mitochondrial cytochrome b region (1138bp) which has 10 haplotypes.2. Twenty-seven complete sequences of the mtDNA D-loop control region (879bp) were sequenced, and there are 9 haplotypes in this region.3. Based on the above results, we considered that the individuals living in the different altitude can not be differentiated by sequencing and that there is only one population in these individuals rather than three ones.There are another two species of the genus, G reticulatum and G akhtari. We didn't find any reports about them. The results of this study can offer some theoretical foundation for further study of them.