Study On The Inhibiting Effect And Mechanism Of The Mixture Between Plant Extract Ts-39 And Primethanil Against Botrytis Cinerea Pers

The appropriate extracting method for Ts-39 was tested based on the inhibiting effect to mycelium growth of Botrytis cinerea Pers. The result showed that the appropriate solvent is alcohol, the appropriate proportion between plant material and solvent is 1:10 (w/v) and the optimal extracting time by ultrasonic is 30 minutes.The synergistic effects of the mixtures between Ts-39 and fungicide Primethanil, Procymidone and Chlorothalonil in different proportions (including 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8,1:9,0:10) against Bortrytis cinerea Pers were tested by using the method of Horsfall. The result indicated that the synergistic ratios (SR) of the most treatments between Ts-39 and Primethanil were better than that of Ts-39 with Procymidone or with Chlorothalonil. The synergistic effects of the mixtures between Ts-39 and Primethanil were tested on detached leaves and tomato plants in the following steps. The results showed 8T/2A had the strongest virulence and its CTC was 137.04. So the proportion of 8:2 (8T/2A) is the optimal synergistic mixture ratio, which could be further developed and studied.The induced resistance of Ts-39 against Botrytis cinerea Pers on tomato plant at the eighth-leaf stage of seedlings was tested. The result indicated that resistance was produced on the upper leaves of tomato plants against Botrytis cinerea Pers by pre-spraying Ts-39 on the lower four leaves at different times ahead of inoculation. The inhibiting effect was significant when the whole plants were treated before challenge inoculation and the variation of the inhibiting effect of Qsj and sj treatments was similar. The result showed that plant extract Ts-39 can induce resistance against the fungi on tomato plant.The enzyme activity of the fifth leaf from the bottom on tomato plants, including POD, PPO and PAL, were tested when the plants were treated on the lower four leaves by water, the plant extract and the conidia suspension of B. cinerea Pers seperately. The results were listed as follows: The level of the three enzymes' activity was stable after the plants were inoculated by water after 1 to 9 days; The activity of POD increased greatly at 6th day after inoculation of conidia suspension of the pathogen, while the activity of PPO and PAL always maintain lower level in contrast to the level of water treatment; Ts-39 can induce the activity of the three enzymes to increase significantly in contrast to the level of water treatment. The activities of POD and PPO increased to the highest level at the 7th day after treatment. The level of the PAL activity reached peaks at the second day and 5th day after treatment.