Research Of Rice Waxy Gene Promoter's Application In The Transformation Of Maize And Consruction Of Bkt Gene Expression Vector Of Maize

Tissue-specific promoter can drive foreign genes'expression in plant's specific tissue and specific time.Rice wary gene encodes the starch synthesizing enzyme combining to starch,it controls the synthesizing of amylase in the farina,endosperm and megaspore.The rice wary gene promoter and GUS gene were linked and transformed to maize type II callus. Many blue spots were observed in the transgenic maize callus by histochemical staining, however there was no in the comparision.45 days later ,the blue sports can still be seen by histochemical staining.The results suggest that rice wary gene promoter an drive foreign genes'expression in maize endosperm. Furthermore, foreign genes'expression in the different part of maize will be checked to see if it is exist and if it is strong or unconspicuous by steady heredity. Early nptII gene was used for screening of the transgenic maize.Now it is found that there is natural resistive in the maize tissue to the nptII gene.So nptII gene is gradually substituted by hpt and pat gene. It is proved that maize calli was sensitive to Hygromycin, 5μg/ml Hygromycin will obviously restrain the growth of maize callus.With the increasing of Hygromycin concentration,the death of maize calli increases.When the Hygromycin concentration is 50μg/ml, all the maize calli will die.The half death is 20μg/ml. There is much Zeaxanthin in the maize but not find Astaxanthin.It is known from the synthesizing way of isoprenoids that Zeaxanthin being catalyzed by bkt gene may be made into Astaxanthin. This research bkt gene was linked with CaMV35S promoter or rice waxy gene promoter...