| Hordeum brevisubulatium(Trin.)link is a kind of perennial forage grass belonging to gramineous type barley genus, alias Wild barley. Its tillering ability and cold endurance and salt-tolerance capacity are strong. By this,We can cultivate some salt-resistant new lines with more other excellent characters. 64 self-bred lines which have been planted 3 years are studied. We have selected 9 salt-resistant mutant lines (Line No 7-5, Line No 7-12, Line No 8-4, Line No 8-7, Line No 9-4, Line No 10-2, Line No 10-6, Line No 11-2, Line No 12-7) with prominent agronomic traits and basically accordant growth; Then, we study the culm lengh, tiller number, fresh weight, dry weight, stem/leaf ratio, single plant seed-yield and 1000-grain weight of these mutant lines and the control group(CK). We make variance analysis and multiple comparisons to these statistics. the results show that: 1) on culm lengh, fresh weight, dry weight: Line No 7-12, line No 8-7, line No 10-6 are better than the control group(CK); 2) on stem/leaf ratio: line No 10-6 is better than the control group(CK); 3) on tiller number, single plant seed-yield, 1000-grain weight: the 9 mutant lines and the control group(CK) are basically the same.From the above,we can suggest: On culm lengh, tiller number, fresh weight, Line 7-12,line 8-7,line 10-6 are apparently different from the control group(CK) in genetics; On stem/leaf ratio, line 10-6 are apparently different from the control group(CK) in genetics; on tiller number, single plant seed-yield, 1000-grain weight, the 9 mutant lines and the control group(CK) are basically the same in genetices. To promote the process of breeding, By RAPD-PCR, from 200 random primers,we have screened out 11 primers which can produce stable polymorphic difference among the 9 mutatious lines and the control group (CK). We have identified characteristic bandings of every lines which can be used to identify this very line. The results of identification of the mutant...
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