Cloning Of The Relate Gene Fragments And Analysing Of The Composition Of The Antagonistic Bacterial Material To Fungi Disease Of The Cotton

Cotton is the crop that provides industrial raw and has largest cultival area all over the world. China is one of these counties which provide amount of cotton,the condition of cotton growth and production is very importart to the development of nation economy. There are many diseases which endanger the growth of cotton, mainly, among them,cotton verticillium wilt and Fusarium Wilt are particulary serious. Cotton verticillium wilt is one of the most crushing disease to cotton. Pathogen of cotton verticillium wilt is Verticillium Dahliae Kleb (abbreviate Vd) which belongs to Deuteromycotina, Verticillium ,It distributes widely, can live inside many kinds of propagate, and it also has many ways to transfer,surviving for a long time, it is a disesea tranfer by soil .and it is extremely difficult to prevent and cure, the cancer known as cotton.Cotton Fusarium Wilt is one of the most serious quarantine disease to the cotton.For many years, scientists have done a large amount of research work in biological control of the cotton verticillium and Fusarium Wilt, although great progress has been gained,problems still exist.The main problem is that biological control measure in verticillium and Fusarium wilt is not steady enough,theret still no succeeded antagonism applied in produce and popularize .There are biological control commodities for other crops,but still no one for cotton at present.Through antibiotic trap and plate antagonists, 85 bacteria which can resist cotton Verticillium and Fusarium wilt gained by collecting the soil that around root of hot pepper , cucumber , tobacco from Hunan, Guangxi,Beijing,ect.use get.By further reaserch 15 bacterial strains with better resisting function have be gained.Amony them,the best 2 pieces (A6 and A7) have been choosed for further research.The resisting rate of A6 to cotton Verticillium and Fusarium wilt are 46.3% and 56.7%, andA7 is 44.7% and 48.2%.By testing the function material of A6 and A7 after cultivated 18 and 20 hours.A6 and A7 are tentatively confirmed as Bacillus Amyloliouefaciens, By 16srDNA and Microlog-BiologTo understand antibiotic mechanism of bacterial strain,antibiotic material in A6 and A7 has been studied and the result shows:Bacterial strain,volatility extract, non-volatility extract ,protein extract and non-protein extract all have function on resisting to cotton Verticillium and Fusarium wilt, among them it is non-volatility extracts that have the most obvious function . That resisting rate of Volatility extracts of A6 to...