Studies On The Relation Between Pollen Abortion And Apoptosis Of Young Spike And Anther In Rice

Objective: Try to comprehend the relation between pollen abortion and apoptosis of young spike and anther in rice. So a mechanism of fertility alteration could be analyzed with the views of apoptosis. The mechanism can provide academic references for breeding of and multiplication male sterile rice, breeding of hybrid rice and the high yielding culture. Ultimately the potentiality and the heterosis of hybrid rice may be highly explored and used.Methods: By using methods of pollen sterility examination under microscope, bagged seed and seed setting, veracity of drawing materials was insured. By using techniques of DNA agarose electrophoresis and investigation of paraffin section, an extensively cytological study was conducted on the young spike and anther development of 4002B and 4026B, two normal cultivars; 4001A and 4025A, two three-line male sterility rice; peiai 64S and zhu 1 S,two two-line male sterility rice. Results:1 DNA fragmention can be detected in the young spike and the anther of rice. The results provide further evidence that DNA during the apoptosis of rice cell did not formed a characteristic "ladder" but "continuous electrophoregram" on agarose gel electrophoresis.2 Apoptosis can't be detected in the young spike of fertile rice plants, including conventional rice plant and fertile plant of male sterile rice line. But it can be detected in the anther of them during the pollen plenum stage.3 Apoptosis can be detected in the young spike and the anther of male sterile rice...