The Studies On Anther Cultural Technological System Of Cabbage

Anthers from 6 genotypes of cabbage were cultured. The effects of genotype and medium type were analyzed in anther induction and differentiation culture, and the ploidy identification and transplanting methods of regenerate plants from cabbage anther culture were studied. Meanwhile, using hybrids of F2P192 and F2P194, factors were studied that had effects on induction culture of cabbage anther, such as hormone, active carbon, pretreatment, culture condition, material positions and sucrose concentration in cultural medium. The results showed that: 1. Genotype was main factor that had an effect on success in cabbage anther culture. It was significant difference in induction rate of anther culture. Hybrid of F2P192 of generation F2 had the highest induction rates, which was 69.17%; in contrary, hybrid of F1S13 of generation F1 had the lowest induction rates, which was 35.83%. Genotype also had an effect on differentiation rate. There were only hybrids of F1P194 和S3P202 that could differenate green plantlets among 6 genotypes, and differentiation rates were 9.26% and 1.61%, respectively. There was a higher induction rate for generation F2. 2. The types of medium affected the induction and differentiation culture of cabbage anther. As basal medium B5 medium was fit for induction culture; 1/2MS medium supplemented with BA1.0mg/L, KT1.0 mg/L and NAA0.1 mg/L, as differentiation medium, was satisfied with inducing callus to differenate green plantlets, which differentiation rate was 2.27%. A proper content of KT could make for differenating shoots. 3. The regenerated plantlets from anther culture were easy to grow roots. The rate of inducing roots was 100% in the medium inducing roots which was 1/2MS medium supplemented with KT2.0 mg/L, NAA0.1 mg/L, 0.8% Agar and 3% Sucrose. Regeneration plants could be easy to adapt to natural environmental conditions by methods of multi-step transplant, which survival rate was 100%. 4. The ploidy of regeneration plants could be identified by observing length of stoma guard cells quickly and exactly. 5. The analysis of range value showed that, 2, 4-D had an important effect on anther induction culture. Hormone composition of 2, 4-D2.0 mg/L and KT2.0 mg/L could be better to promote induction culture among all hormone compositions. Active carbon couldn't promote anther culture, and induction rate of active carbon treatment was lower than contrast treatment slightly. The treatment that buds were pretreated with 4℃low temperature during 72 hours before anther culture and anthers were cultured darkling with 30℃high temperature during 72 hours at initial stage, could obtain better inducing result. Its induction rate was 25%. At the same time, the analysis of range value showed that, dark culture with high temperature affected induction rate importantly. There was no significant influence on induction culture which materials were getten from different position of the same plant at same growth period, and the apical predominance wasn't showed in anther culture. The induction rate of 6% sucrose treatment, which was 30.56%, was significantly higher than which of 3% sucrose treatment. Thus, high concentration sucrose could stimulate germinating microspores and increase induction rate.