The Antitumor Effect On Transplantable S₃₇ With Newcastle Disease Low Virulent Strain Vaccine And SW Or Poly I: C At Rats

The paper has three parts. The first part includes the preparing of Newcastle disease low virulent strain vaccine, studying it's stability and security, detecting it's antibody level in order to know the accurate content of virus. And acquire the proper cure dose.The second part checks the immunology targets on antitumor cooperation of Newcastle disease low virulent strain vaccine with swainsonine (SW) and polyinosinic and provides the cooperation antitumor effects of immunology in the body of animal by the Newcastle disease low virulent strain vaccine with the two others.The third part detects targets of the cooperation antitumor effects of pathology in the body of animal by the Newcastle disease low virulent strain vaccine with the two others and Provides direct bases for the vaccine cure tumor .Main research contents include: 1.The rats were divided into three groups injected three different vaccine concentration three different times.Antibody level, security and toxicity were detected .Emagglutination inhibition test shows that the original vaccine group has good effect. 2.We built cancer molds by transplanting Sarcoma 37 cells into rats left forelimb oxter endermic. From the first day, injects the original vaccine 0.2 milliliter into groupⅠ, groupⅡand group Ⅲseparately every two days, group Ⅱhypodermic polyinosinic acid-polycytidylic acid 100μg/one/d every four days from the first day. Group Ⅲpoured swainsonine into the stomach of the rats, and the doses of swainsonine was 8.1mg/kg/d every two days. MTT method was used to observe the T lymphocytes proliferation after 0d, 4d, 8d, 12d and 16d. The swallow percentages of peritoneal macroPhages were determined after 0d, 4d, 8d, 12d and 16d. Antibody levels were detected after 0d, 4d, 8d, 12d and 16d. .The result showed, Antibody levels, group Ⅰ, groupⅡand group Ⅲcompared with the contrast group on 4d,8d,12d,16d is especially different ﹙P<0.01﹚. Ⅲgroup compared with group Ⅰdifferently﹙P<0.05﹚. The SI result showed group Ⅰ, groupⅡand group Ⅲcompared with the contrast group on 8d,12d is esPecially different ﹙P<0.01﹚. The SI result showed groupⅠ, groupⅡand groupⅢcompared with the contrast group on 4d,16d is different ﹙P<0.05﹚. GroupⅢcompared with groupⅠand groupⅡis especially different on 12d﹙P<0.01﹚. The swallow percentages of peritoneal macrophages showed group Ⅰ, groupⅡand groupⅢcompared with the contrast group on 4d,8d is especially different ﹙P <0.01 ﹚. GroupⅠcompared with the contrast group on 4d is different ﹙P<0.05﹚. GroupⅢcompared with group Ⅰa nd groupⅡis especially different on 8d﹙P<0.01﹚. 3.We built cancer molds by transplanting Sarcoma 37 cells into rats left forelimb oxter endermic. From the first day, injects the original vaccine 0.2 milliliter into groupⅠ, groupⅡand group Ⅲseparately every two days. group Ⅱhypodermic polyinosinic acid-polycytidylic acid 100μg/one/d every four days from the first day. Group Ⅲpoured swainsonine into the stomach of the rats, and the doses of swainsonine was 8.1mg/kg/d every two days. Weight , the ratio of tumor inhibition were detected on 0d, 4d, 8d, 12d and 16d. We observed the pathology changes by optics microscope. The result for ratio of tumor inhibition showed that group Ⅰ, groupⅡand groupⅢwere 23.5%,38.1%and 44% on 4d; that groupⅠ, groupⅡand group Ⅲwere40.6%, 8.3%and 56.2% on 8d; that groupⅠ, groupⅡa nd groupⅢwere33.89%,34.75%and50.36% on 12d; that group Ⅰ, groupⅡand groupⅢwere33.39%,50.16%and 37.46% on 16d. GroupⅠand groupⅢwhich ratio of tumor inhibition kept on high levels and kept stability, we believe in the experiment of antitumor effects the both have good effects. EsPecially, groupⅢhad a high effect on antitumor.While ratio of tumor inhibition of group Ⅱis instability, the antitumor effect has to be studied infuture. In the experiment of pathology observation, group Ⅲhad badly fatty degeneration and large area putrescence in tumor tissue, and the putrescence area was meshwork shape, and cut the tumor cell into some grids .while group Ⅰshowed worse than group Ⅲ.