Studies On Anti-Pregnancy Of Toosendanin In Pregnant Mice At Early Stage

Virgin female mice weighing 22±2g were superovulated, and then mated with male mice. The acquired pregnant mice were divided into 6 groups for embryotoxicity research of toosendanin on fetuses of mice in 2-Cell, Morula, Blastocysts Stages. Control group was set up in each of these three stages. Every pregnant mouse of treated and control groups in the three stages was given the dose of 1/30 LD50 toosendanin and same amount of PBS 2 times by ip injection respectively. Superovulation need not be carried out for embryotoxicity study of toosendanin in Implantation Stage, but each pregnant mice in treated and control group was given the dose of 1/30 LD50 toosendanin and PBS 3 times by ip injection respectively. Finally, the number and configuration of embryo in uteri of all groups were counted and examined. Results: Total embryo of treated group In 2-Cell, Morula and Blastocysts Stages had no significance difference comparing with corresponding control group(P > 0.05); Number of normal and abnormal embryo of treated group in 2-Cell Stages had no significance difference comparing with control group either(P > 0.05); Number of normal and abnormal embryo of treated group In Morula and Blastocysts Stages had great significance difference comparing with corresponding control group(P < 0.01). Due to injection to the treated group 3 times in Implantation Stage, almost all the fetuses in uteri had been melting, and counting of them could not be performed while healthy fetuses could be seen and counted clearly in control group. There are only mild histological changes in maternal heart, liver, lung, kidney and uteri in the all treated groups. All above means that injecting ip a small amount of toosendanin to pregnant KM mice may has special embryotoxicity.