Effects Of Total Flavones Of Epimedium On Tissue Damage Induced By Acute Cold Exposure And Antioxidative Activity In Rats

90 Kunming rats, being clinical healthy, were divided into five groups, 18 for each group. Group 1(normal control group), rats were given for 4 weeks with physiological saline 2ml/d by ip; group 2,rats were given for 4 weeks with EF (1ml/d, ip); group 3(model control group), rats were given for 4 weeks with normal saline 2ml/d, group 4 and 5,the experimental groups, were given EF 1,2ml/d by ip, continuously for 4 weeks. After 24 hours of the last administration, rats of group 1 and 2 were kept in regular temperature(18℃) and group 3 to 5 were exposed to -20℃for 40 minutes. Tissue and serum samples were taken after 1 hour, 10 hours and 20 hours of cold exposure. The SOD activity, MDA contents, GSH-Px activity, XOD activity, GPT activity, BUN and Scr contents were measured in rats. The results were as follow: Compared with the group of normal control, in group 2,the activity of SOD in tissues and serum were increased and the contents of MDA were decreased; the GSH-Px activity in cardiac muscle were increased; the contents of BUN and Scr and the levels of XOD and GPT in serum were fallen. the contents of BUN at 1 hours in serum, the contents of Scr at 20 hours and the contents of MDA at 10 hours and 20 hours in serum and the levels of XOD at 1 hours in serum were different from nomal control group(P<0.01).in group 3,the activity of SOD in cardiac muscle,except for 10 hours in liver, in renal and serum were decreased significantly(P<0.05 or P<0.01);the GSH-Px activity in cardiac muscle were decreased significantly(P<0.01); the contents of MDA except for 1 hours in liver, in renal, in cardiac muscle and serum were increased obviously(P<0.05 or P<0.01);the contents of BUN at 10 hours in serum , the contents of Scr in serum , the levels of XOD and GPT in serum were improved significantly(P<0.01).