Zucchini Yellow Mosaic Virus Coat Protein Mediated Resistance For Watermelon Viruses

The genes derived from viruses or plants have been shown to be resistant to plant virus infections. It is the main strategy of resisting virus by biological engineering. Coat protein mediated resistence has been the earliest and proficient technology. But the mechanism of resistance (RNA-mediated or protein-mediated) has not yet been completely understood. RNA-mediated resistance shows several advantages over protein-mediated resistance. Such as biological safety and usefulness. It has been widely studied in recent years. This research is as the following :l.The construction of plant expression vector of the coat protein gene of Zucchini yellow mosaic virus from china. The experiment was conducted with vector ZCP-87 containing the coat protein gene of Zucchini yellow mosaic virus isolation of China. The vector liased to plant expression vector pBin438 (recombinant plastid designated as pBZCP5). The PCR and double digestion test showed that the coat protein gene was constructed into plant expression vector. The experiments reported here are basic steps in transgenic research for resistance to ZYMV.2.ZKM watermelon as tested material, the results show that the seeds of watermelon were sterilized as follows: firstly, rinsing with 70% alcohol for 30s; secondly, sterilizing with 0.1% HgCl2 for 5min and washing with sterile distilled water about 3 to 5 times; lastly, putting these seeds on 0.8%ager media. It is the best germination medium. The seeds were cultured in dark room for three days and then transferred into shiny room. One week later, the sound seeds sprouted. Using Cotyledon as explant, the medium suitable for Cotyledon producing adventitious buds was MS+2.0mg/L BA+0.1 mg/L IAA, and the rate of differentiation has reached 97.7%. The adventitious buds stretched fast in the medium of MS+KT(0.2mg/L 0.5mg/L 1 .0mg/L). The selection concentration of Kanamycin is 100mg/L.3.The aim gene was introduced into ZKM plants via Agrobacterium tumefactions-mediated transformation. The transformed tissue was selected in the...