Function Analysis Of PSD1,a Key Gene For Floral Organ Development In Rice(Oryza Sativa L.)

psd1(pistil-stamen degeneration) is a mutant of the key gene that controls the development of sexual organs (pistil and stamen) in rice(Oryza sativa L.), which was found in a double haploid (DH) popular from the cross between Taiwanjing and Gui630 derived by anther cuIture.The phenotype of psdl mutant plants seemed to be normal. However, the spikelets of psdl were larger than the normal ones, and its stamens and pistils were totally degenerated, with many lays of glumes instead. Functional characterization of the gene PSD1, would be very helpful for deep understanding of the mechanisms of floral development in rice (Oryza sativa L.).psdl candidate gene was obtained by fine mapping, but it needs to have a further test .The wild-type isogenic lines of psdl candidate gene (wild type) is transferred into its mutant and wild-type and Nipponbare to previously identify it and investigate its function by genetic complementary test and overexpression, at the same time , discuss its application prospects. In this study, Major results are as follows:(1) The complete open reading frame of psdl candidate gene was cloned, and connected with maize ubiquitin promoter and constructed on plant binary expression vector pTCK.303-.PSD1.(2) Mediated by Agrobacterium, PSD1 was introduced into psdl mutants and normal plants and Nipponbare, respectively. And then twelve transgenic rice plants were obtained and seven were further determined to be positive through PCR assay (58.3%).(3) And now, there are more than ten regeneration plants through...