| Lysine is the first restrictive amino acid of rice protein, which plays a critical role in defining the nutrition quality of rice. The utilization efficiency of rice protein can be improved by increasing rice lysine content. In Fujian Province every year 7,000,000 tons of rice grain is produced and increasing rice lysine content by 10 percent equates adding 49,400 tons of fine quality protein, i.e., 371,400 tons of eggs. For a long period of time conventional rice breeding has achieved little progress in improving rice quality. Genetic engineering is providing a effective new way to improve the seed protein quality of rice grain.Lysine-rich protein of Psophocarpus tetragonolobus(L.)DC is rich in lysine, which reaches 11%, and its Ser, Val, He contents are also high. The lysine content of rice protein is about 0.31%. In this research Taigen9 which spreads widely in tropic and subtropic areas of the south of China, was used as receptor for genetic transformation. Agrobacteria-mediated transformation was adopted to transferring the high lysine gene into Taijing and molecular determination was also conducted. The main results of this research are as follows:1. Establishing Taijing 9 high-frequency regenerating systemThe effects of explant, media, ingredient of different hormones and generation number of subculture on Taigeng 9 callus induction and regeneration were studied and a high-frequency regenerating system for Taijing 9 was established. The best explant for induction was juvenile embryo 12-15 days after pollination; The best callus-induction and subculture media was NB; The best hormone ingredients of differentiation media was 0.05mg.L-1NAA+2.00mg.L-16-BA; During Establishing high-frequency regenerating system, adding 2.0mg.L-1 2,4-D was the most suitable concentration for callus cultivation of Taigeng 9. |
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