| Sterilized seedlings of strawberry cultivar 'Toyonaka' and 'Zoji' in Sichuan were used as materials. On the basis of previous work, adventitious buds induction with 4 kinds of combinations of PGR (plant growth regulators) and effects of dark cultivation, concentration of AgNO3 on adventitious buds regeneration of leaves from 'Zoji' were studied. The effects of different kinds of antibiotics were studied on the respective medium with the highest rate of shoot regeneration. The results were as follows:First, by comparing the rates of adventitious buds regeneration and their quality in four treatments, it came to the following conclusion: MS medium supplemented with 2.0 mg-L-1 TDZ and 0.6 mg-L-1 IBA was most effective for 'Toyonoka' with the regeneration rate of 88.96%, while the best combination of PGR for 'Zoji' was 2.0 mg L~-1 TDZ and 0.8 mg L~-1 IBA with 21.67% regeneration rate of adventitious buds.Second, dark cultivation could improve callus induction of 'Zoji', but the callus got brown easily after being exposed to light. The longer the dark period was, the more seriously the callus got brown. In comparison with dark-free, the bud regeneration of 'Zoji' was inhibited obviously by dark-cultivation and got more and more significant with the dark-cultivation prolonging.Third, for Zoji, the addition of AgNO3 varied from 2.5 mgL~-1 to 10.0 mg L~-1 to the medium could bring forward the date of shoots' appearance from 7 to 12 days. What's more, the rate and the number of adventitious buds regeneration per leaf were improved and the effect was more obvious with the concentration of AgNO3increasing. The rate of shoot regeneration could reach 70% with better shoot quality when the concentration of AgNO3 was improved to 10.0 mgl/'.If the concentration of AgNO3 was above 10.0 mg-L"1, the emergence of adventitious bud would be inhibited.Fourth, the shoots' appearance was delayed from 10 to 15 days with the addition of Cef, Amp, Pen G and Crb. Pen G had no effect or slight effect on callus induction, while Cef, Amp and Crb inhibited callus induction significantly and its inhibition improved with the concentration increasing. Cef had the least inhibition and Crb had the most on the adventitious buds regeneration with the same antibiotics concentration. When the concentration was at 25 mg-L"1, Amp, Crb and Pen G had inhibited the rate of adventitious buds regeneration significantly or very significantly, while Cef inhibited shoot regeneration of leaves from strawberry 'Toyonoka' or 'Zoji' significantly at 50 mg-L"1 and 150 mg-L"1 respectively.Fifth, 'Toyonoka' and 'Zoji' were sensitive to Km and Neo. Both Km and Neo inhibited callus induction very significantly and completely inhibited adventitious buds regeneration at low concentration (5 mg-L"1). The callus induction of 'Toyonoka' was completely inhibited when Km was at 10 mg-L"1 or Neo was at 15 mg-L'1. And the same happened to 'Zoji' when Km was at 15 mg-L"1 or Neo was at 30 mg-L"1. It was discovered that Km caused some leaves of 'Toyonoka' to turn yellow when its concentration was at 5 mg-L"1, and the toxicity enhanced with its concentration increasing. Km made 'Toyonoka' leaves all white and die within three weeks at 30 mg-L"1. However, when the concentration of Neo was improved to 30 mg-L"1 the leaves of 'Toyonoka' and 'Zoji' kept green although the frequency of callus induction and adventitious buds regeneration were zero.
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