Synergy Of Recombinant Enhancin Of Trichoplusia Ni Granulovirus And Pseudaletia Separata Entomopoxvirus

Abstract: The synergy of recombinant enhancin of Trichoplusia ni granulosis virus,which was expressed in Escherichia coli and named as P96, while the synergy of recombinant enhancin of Pseudaletia separata entomopoxvirus, which was expressed in Escherichia coli and named as P39, the two synergies on Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) against the larvae of H.armigera were studied. Eleven days after inoculation, the LC50 for HaNPV+P96 were 3.89× 10⁴ and 3.47 × 10³ polyhedra inclusion body (PIB)/mL respectively. Within the concentration of 1.6 × 10⁴¹.6 × 10⁶ PIB/mL, the values of LT ₅₀ for HaNPV+P96 were reduced by 0.3¹.8d compared with those for HaNPV. Three-dimension surfaces of cumulative mortality, as affected by both time and dose, were derived by a time-dose-mortality model. The results showed that the virulence of HaNPV was enhanced remarkably by P96. Eleven days after inoculation, the LC₅₀ for HaNPV and HaNPV+P39 were 13831 and 1082 polyhedra inclusion bodies (PIBs)AnL, respectively. Within the concentration of 1.6×10³¹.6×10⁶ PIBS/mL, the values of LT₅₀ for HaNPV+P39 were reduced by 0.5-2.7d compared with those for HaNPV. Three-dimension surfaces of cumulative mortality, as affected by both time and dose, were derived by a time-dose-mortality model. The results showed that the virulence of HaNPV was enhanced remarkably by P39.