Screening And Identification Of The Genes Transactivated By Plant Activator Protein By CDNA Microarry

Plant activator protein is a novel proteinaceous elicitor originated from Alternaria spp. The plant activator protein has no virulence and it can improve the activity of plant dehydrogenase, peroxidase, phenylalanine ammonialyase and proline. It improves plant immunity by activating plant immunity system, in order to advance the growth of plant.The use of Gene chip has become a standard tool for parallel gene expression analysis. Recent improvements in DNA microarrays enable whole-genome expression analysis, and thus open a new avenue for studies of the composition, dynamics, and regulation of the transcriptome in plants. Northern blotting, differential display are available, however, these methods each have their disadvantages, which render them unsuitable if large numbers of expression products have to be analysed simultaneously, while the gene chip proves a high-throughput tool for an unbiased screening of differentially display gene. DNA microarrays are being increasingly used in plant biology research for the parallel analysis of gene expression. In this paper gene expression profiling was employed to analysis genes expression in Oryza sativa induced by plant activator protein, which brought some new clues for studying the action mechanism of plant activator protein.Objective: Gene expression profiling was employed to analysis genes expression in Oryza sativa induced by plant activator protein, a novel proteinaceous elicitor originated from Alternaria spp. Methods: cDNAs from Oryza sativa treated with plant activator protein solution (2μg /ml) and that from control group were labeled with fluorescent dye Cy5 and Cy3 respectively. The two fluorescent cDNA probes were mixed and hybridized to gene chips contains 10,368 Oryza sativa genes, and the chips were scanned by biochip scanning system. Differential expression of genes was analyzed by the ratio of Cy5 and Cy3 signal intensities. Results: 97 genes of differential expression were screened out. 4 genes were up-regualated and 93 genes were down- regulated. Conclusion: First, this study demonstrates that cDNA microarrays technology was successfully used to screen the genes differentially expressed by plant activator protein in Oryza sativa, which brought some new clues for studying the action mechanism of plant activator protein. Second, these differentially expressed genes are involved in many different biological processes and molecular functions, such as photosynthesis, signal transduction, regulation of gene expression, metabolism, development, ribosomal...