The Study Of The Apoptosis Of The Chicken Bursa Of Fabricius Cell And Thysum Cell Induced By REV And ALV-J

In order to discuss the SPF chickens thymus cell apoptosis induced by reticuloendotheliosis(REV) and avian leucosiS virus of subgroup J(ALV-J),we applied for in situ cell death detection and the H.E dyeing,also,we observed with electronic microscope.The results indicated that both REV and ALV-J can induce the SPF chickens thymus cell apoptosis and when they were coninfected with two virus the SPF chickens thymus apoptosis level was more serious than single infection.We could find that apoptosis cells were more than the death ones.All these showed that apoptosis is the main reason that caused the SPF chicken thymus constrain.1.Pathological model was established in commercial broilers at one-day-old that inoculated by avian leukosis virus subgroup J(ALV-J) strain NXO101 , retiuloendotheliosis virus(REV) strain SD9901 and ALV-J+REV.Primary study showed that body weight of broilers infected with ALV-J or REV,compared with the control,was significantly depressed and antibody response to NDV vaccine was inhibited. In addition,these broilers were susceptible to bacterial agents,such as colibacillosis,causing higher lethality rate in early time.All that above was even more serious in broilers infected with ALV-J+REV.2.Apoptosis of iymphocytes in thymus and bursa was detected by light microscope, electron microscope and terminal-deoxynueleotidyl transferase UIP nick end labeling(TUNEL).The results showed ALV-J could induce incoordinate apoptosiS of lymphocytes in thymus,bursa,which was mainly found in early time(in two weeks).And the apoptosis was much...