| The disease of avian embryo is a synthetical disease which can cause the increase of embryo death and decrease of incubation rate and is fatal to economy. This disease appeared in the 2nd,3rd and 5th of one large-scale AA broiler breeder farm(1.2 million AA broiler breeder,12 sub-farms),with the symptom of embryo death, increase of weak chicken ,decrease of incubation rate and inspritory symptoms in chicken,since June, 2004. The infection rate of E.coli and NDV increased, and the growth was stunted in 30 days chicken, and this phenomenon didn't appear in other sub-farms. So it was concluded that this disease was related to broiler breeder. To further study the cause of disease of avian embryo in this large-scale AA broiler breeder farm, epidemiological investigation pathogenic studies and PCR to main pathogens were done, supplying the theoretical foundation to the prevention of avian embryo in large-scale AA broiler breeder farm.The research divide three parts:Part One: Pathogenic studies on the diseases of avian embryo in AA broiler breederA kind of disease appeared in one large-scale AA broiler breeder farm in Shandong province with the symptom of embryo death, increase of weak chicken, ineffective immunity, stunted growth of 30 days chicken since June, 2004. Antibody in serum was tested to the broiler breeder, 1d chicken and the commercial chicken, it was showed that the average positive rate of CIAV, VAV were 4.67% and 3.00%,respectively. The average positive rate of bordetella avium, salmonella, E.coli, mycoplasma were 13.59%,12.69%,8.10%,19.62% and 37.14%, respectively. Bordetella avium(62.60%), E.coli(51.50%), salmonella (26.30%), mycoplasma (26.67%), CIAV and VAV were separated from the organs of the death embryo, weak chicken and the commercial chicken by pathogenic study. It was showed that the co-infection of bordetella avium, salmonella, E.coli, mycoplasma, CIAV and VAV was the major cause of the...
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