Agrobacterium Mediated Transformation Of Low-temperature Induced Transcription Factor CBF1 Gene Into Anthuriun Andreanum

C-repeat/dehydration-responsive element binding factors (CBF1) is a transcriptional activator, which was isolated from Arabidopsis thaliana cDNA using the yeast one-hybrid method by Stockinger's lab in 1997. The CBF1 gene has a typical Apetala 2/ethylene-responsive element-binding protein (AP2/EREBP) domain for recognizing and binding the C-repeat/ dehydration-responsive element (CRT/DRE) of cold-responsive genes (cor). Jaglo-Ottosen reported that over-expression of CBF1 gene induced cor gene expression and increased the freezing tolerance in plant. At present, there are many reports related to the genetic transformation of CBF1 to improve the freezing tolerance of plants. Many reports indicated that over-expression of CBF1 gene affected the expression of several target functional genes, consequently improving the tolerance of plants to drought, high-salt and cold stress. Thus, it may likely become an effective strategy to enhance the tolerance to low-temperature for those tropical plants.Anthurium andreanum is a valuable ornament of monocotyledons and lives in tropical rain forest. It is attractive for human because of it's longer anthesis and it's attractive wattlew and colorful blossom. It survives at wet and warm habitat of an optimal temperature between 20 to 28℃. It can not grow at low-temperature area such as the north of China. Therefore, we attempted to introduce CBF1 gene into Anthurium andreanum, so as to improve its tolerance to low temperture.At present, there are many methods for gene transformation of plants,...