Studies On Molecular Ecology Of Pinus Sylvestris Var. Mongolica Population In North Part Of Great Xing'an Mountains

Pinus sylvestris var. mongolica is a geographical variation of Scots pine, and it distributes in Great Xing'an Mountains and HuLunbeier sand of Northeast of China. The species has important value in both conservation and research. The mainly processed researches of Pinus sylvestris var. mongolica were in morphological description, ecological characters, and cultivation technique ect.. There were less research in conservative genetics and the correlativity between environmental factors and genetic diversity in natural populations of P. sylvestris var. mongolica. This paper focused on two aspects of the species by using ISSR techniques. One is the genetic structure and genetic diversity in natural populations of P sylvestris var. mongolica , the other is the correlativity analysis of environmental factors and genetic diversity. Primary results of this paper were summarized as following:1. Genetic variation of P. sylvestris var. Mongolica is notable. "The sampled individuals of P. sylvestris var. Mongolic were from four natural populations. By using the inter-simple sequence repeat (ISSR) technique, the genetic diversity and genetic structure of the species were analyzed. 16 generated highly reproducible and stable primers were selected from 60 ISSR primers. Using these primers, 190 discernible DNA fragments were produced, 162 (PPB=85.26%) of them were polymorphic loci, which indicated the high level of population in genetic variation. The result of POPGENE analysis showed, the genetic diversity of P. sylvestris var. mongolica (He= 0.2758, I=0.4162) was higher than other conifers. Genetic differentiation (Gst) among the natural populations was 0.2478 in average by Nei's genetic diversity analysis. Gene flow (Nm) was 0.1648. That means the genetic variation is plainly occurred within natural Mongolian pine populations. The ISSR marks were available in genetic diversity and genetic structure of P. sylvestris var. mongolica.2. The correlativity analysis of the environmental factors and the genetic diversity. Based on the result of further research, we selected 3 main environmental factors (altitude,community type and latitude) to analyze the correlativity. The results showed: the genetic-diversity of natural Mongolian pine population was notably affected by altitude and latitude factors, but the differences were unobvious. Meanwhile, the community type had less influence to the genetic diversity of the populations in the study area.In summary, P. sylvestris var. mongolica was rich in genetic diversity among the natural populations, and there was a higher genetic diversity within populations than among populations. Different environmental factors affected the genetic diversity of natural P. sylvestris var. mongolica populations.