The Interaction Between Myzus Persicae (Sulzer) And Hosts & Tobacco Mosaic Viruses

Recently, Myzus persicae (Sulzer) and tobacco mosaic diseases, the most main pest and diseases, appeared on tobacco and have caused tremendous economic losses on the production of tobacco. Tobacco mosaic diseases were involved in three pathogenes of mosaic viruses Tobacco Mosaic Virus (TMV), Potato Virus Y (PVY) and Cucumber Mosaic Virus (CMV). Among them, CMV and PVY were mostly transmitted by insect vector, Myzus persicae (Sulzer). In order to improve crop arrangement, control pest and diseases, ensure yield and quality of tobacco, the types of mosaic viruses infecting tobacco at Wulong county were identified and CMV was isolated by a series of biochemical experiments. At the same time, the interaction between Myzus persicae (Sulzer) and these viruses, host plants were evaluated in this paper. The main results are summarized as follows.1 The types of tobacco mosaic virus at Wulong countyThe types of virus infecting tobacco from the field at Wulong county were investigated and identified by the methods of ELISA (Enzyme linked immuno sorbent assay). We discovered that the symptom of mosaic diseases were mainly caused by Tobacco Mosaic Virus (TMV), Cucumber Mosaic Virus (CMV), Turnip Mosaic Virus (TuMV) and Potato Y Virus (PVY). In 26 samples, all of them were infected by TMV, and 73.08 % of samples were infected by CMV. These samples were involved in the co-infection of TMV, CMV, PVY or TuMV. 3.85% of samples were infected by mixture of TMV and CMV, 15.38% of samples were caused by mixture of PVY and TMV, 23.08% of samples were caused by mixture of TMV, PVY and CMV, and 15.38% of samples were caused by mixture of TMV, TuMV and CMV. The co-infection of TMV, PVY and TuMV is 11.54%. The co-infection of TMV,CMV, TuMV and PVY is 30.77%.2 The isolation and identification of Cucumber Mosaic Virus (CMV)CMV infecting tobacco was isolated using indicating plant (Nicotiana glutinosa) and spot host (Chenopodium amarauticolar), and was identified by a series of indicating plants and indirect ELISA. There were different symptoms on different indicating plants. The symptom of mosaic was presented on Nicotiana tabacum, Nicotiana glutinosa, Nicotaina sumsam, K326, Cucumis floridana and Citrullus vulgaris, the symptom of spot was presented on Chenopodium amarauticolar, Chemopodium qunoia and Comphrana globosa, and the symptom of yellowing-spot was presented on Datura stramonium. The OD of virus sample was > 0.5, and the ratio between virus sample and negative control was > 2. From the results, it could be inferred that the isolated virus was CMV.