Analysis Of Genetic Diversity Of Radish Germplasm Resourse Using SSR And Establilashment Of The Primary Core Collection

Radish(Raphanus sativus L.) is an important vegetable and the germplasm resources are very abundant in China. The study on the genetic diversity of radish can benefit the conservation,classification, identification, genetic enhancement and effective utilization of the radish germplasm resources. Molecular marker, which can detect the genetic diversity at DNA level, is a powerful tool to study genetic diversity.SSR markers have been popularized for their simplicity and rapidity.In this study, the genetic diversity in 47 radish accessions from different countries or regions was assessed using SSR markers, and the genetic relationship among the accessions was revealed by systematic clustering analysis and principal coordinates analysis at various level. continuousWith the development of society and economy, and modern agriculture,especially widely extension of hybrid varieties,the genetic diversity of crop resourses has been destroyed continuously and the genetic base of cultivar has become narrower and narrower. More and more countries pay great attention to the conservation of plant genetic resources. Some gene banks have large collections of plant germplasm, which are often ineffectively managed and seldom accessed by plant breeders. Assessment of genetic diversity on these collections has proved helpful to establish core collections. The core collections are subsets of large collections of germplasm, containing chosen accessions that capture most of the genetic variability in the entire collection. Thus, developing a primary core collection of radish was attempted in order to improve the management and utilization of the radish germplasm collection.The main results were as follows:(1)A total of 24 distinct bands were detected with 6 informative SSR primers among 47 radish accessions. Of them, 16 bands (63.89%) were polymorphic. The average heterozygosity of SSR loci was 0.8750. 47 accessions were clustered into 4 groups based on the SSR data by the method of clustering analysis, and 4 groups by the method of principal coordinates analysis.(2) 441 radish accessions with 12 traits were used as an example for comparing the eficiency of establishing core collection. The core colections were constructed at 5%,10%, 15%,20%,25...