| Bursaphelenchus xylophilus, fungi and Monochamus alternatus coexist in infested pine trees at special period. In pine wilt disease system, there must be complicated interactions among the four, which have been simulated in the experiment. Three tree-inhabited dominated fungus strains, Sphaeropsis sapinea E11, MHS7.3, and Pestalotiopsis microspora M32, isolated from infested Pinus massoniana, are inoculated to artificial pupal chamber of Monochamus alternatus respectively. Effect of different fungi on population dynamics and structure of Bursaphelenchus xylophilus are systematically studied, including structure and dynamics of larva and adult, sex ratio of female to male, Bursaphelenchus xylophilus population dynamics in the process of pupae days and eclosion of Monochamus alternatus, dynamics of nematodes carried by beetles. Meanwhile, effects of M. alternatus and fungi on development of individual nematode are also tested.The PWN population propagating on Pestalotiopsis microspora M32-inoculated host block was the largest, and the PWN population fed on Sphaeropsis sapinea E11 and Sphaeropsis sapinea MHS7.3 were more or less the same. The PWN population fed on Pestalotiopsis microspora M32 and Sphaeropsis sapinea E11 trended to increase, but the PWN population fed on Sphaeropsis sapinea MHS7.3 did decrease. The population dynamic of PWN on fungi-inoculated host block exhibited periodical variation.M. alternatus could carry B. xylophilus whatever in pupae or adult period. When M. alternatus is in pupa stage, third-stage dispersal juveniles (L_â…¢) were mainly carried; and orth-stage dispersal juveniles (L_â…£) were mainly carried in adult stage. L_â…£s were isolated from M. alternatus at the first day of pupae. With the development of M. alternatus, the number of L_â…£ increases greatly. So did the ratio of L_â…£ carried by M. alternatus to total PWN. M. alternatus had no significant influence on PWN population.
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