| Sugarcane is the most important sugar crop and is accounting for more than ninety percent of sugar products in our country. At the same time, sugarcane is one of the best regenerated energy crops for production of fuel ethanol in South China. Sugarcane Yellow leaf Syndrome (SYLS) is a new sugarcane disease discovered in recent years in China and in the world. It causes severely decline in quality and quantity of sugarcane and is responded to variety retrogression. The pathogen of SYLS is now considered to be a virus being named as Sugarcane Yellow Leaf Virus (SCYLV).SCYLV, a new member of the genus Luteoviridae, is a sense ssRNA virus, transmitted by aphids. It is a dangerous pathogen for sugarcane and for gramineous crops, Coat-protein gene encoded by the SCYLV genome is related with the transmition of aphid and play many important functions in activity of virus itself. It has reported that transgenic plant of CP gene can improve plant disease resistance. Because of CP series varieties of sugarcane is sensitive to SYLS, and they are main cross parent in sugarcane breeding in China, it necessary to improve the resistance to SYLV for varieties released in our country. Supported by grant from National 948 project code as (2003-Q06), a pair of specific primers are designed, and the full length of gene fragment from one of Fujian isolation of SCYLV, is isolated by means of RT-PCR technology. The main results are as follows:First, sugarcane leaves with SCYLV syndrome collected in field are used for isolation of RNA. A specific primers used in RT-PCR is designed by ourselves according to the base sequence of SYLV-CP gene published. A target fragment with a size of about 600 bp is isolated by RT-PCR with RNA isolated from diseased leaves as template.Second, the target band is recovered and purified, and then it is cloned into vector of pMD18-T. Then, transformation of E.coli DH5α and the recombinant is selected by the identification of putative recombinants with...
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