The Study On Genotypic Stability And Physiological And Biochemical Changes Of Cucumis Melo Cv. Hetao Transferred Antisense ACC Synthase

Cucumis melon L. cv Hetao is the good regional muskmelon in HeTao area in the west Inner Mongolia. This kind of fruit is fragrant and sweet, and holds great nourishment value. However,the shelf-life of the fruit is short and easy to decay during storage and transportation, which leads to substantial economic loss. In regard of that, good manner should be employed on the fresh-keeping of Cucumis melon L. cv Hetao. Hence, our laboratory had transferred the antisense ACC synthase gene into Cucumis melon L. cv Hetao depending upon the technology of antisense RNA, and we had, as a result, gained the transgenic melon. Researches are made on genotypic stability and physiological and biochemical changes of transgenic melon in postharvest storage period. Results are as follows:1. PCR analysis and phenotypic identification of T₆ generation of transgenic Cucumis melon L. cv Hetao indicated that the antisense ACC synthase gene had integrated into the plant genome.2. The results of agronomical characters of different stains indicated that there were no prominent differences between transgenic and non-transgenic plants.3. The rate of weight loss of transgenic melon was lower than non-transgenic melon during storage. The weight of transgenic melon reduced slower than non-transgenic melon. Change on rigidity of transgenic melon was less marked during storage period. The soluble solid content of transgenic melon was more than non-transgenic melon at percentage of about 11% at the...