Identification And Function Analysis Of Enzymes From Salivary Scretions Of Living Aphids, Sitobion Avenae

The wheat aphid Sitobion avenae is the major pest of wheat in China and many other countries. It is not only as a vector of plant virus diseases, but also making heavily damage to wheat. Aphid saliva is an important invasive component in the interaction of aphids and wheat. So the study on aphid salivary has quite significance, which not only expounds the evolution of plant defense system and the evolutional connection of cross talk of organism, but also provides an important point from which we can systematacially research the coevolution mechanism of wheat, aphids and natural enemies. This paper investigated the enzymes and their function of saliva of S. avenae. The main results are as follows:1. Effect on indirect defense response of wheat treated by S. avenae's saliva extracted with two kinds of artificial dietsThe saliva of S. avenae was respectly extracted by restilled water and 20% sucrose (100mmol/L serine, 100mmol/L methionine, 100mmol/L aspartic acid). The wheat seedlings (Beijing837) were treated with extracted saliva. Then the volatiles were collected using Porapark. Olfactory responses of the four natural enemy species, i.e., Harmonia Axyridis, Coccinella septempunctata, Chrysopa septempunctata and Aphidius avenae to volatiles of wheat treated by extracted saliva were studied."Y"tube olfactory test showed that the volatiles of seedlings treated by 20%sucrose (100mmol/L serine, 100m mol/L methionine, 100mmol/L aspartic acid) attracted the four natural enemy species, but the volatiles of seedlings treated by redistilled water had no effect on them. So it concluded that the diet of sucrose may include the elicitor which can induce wheat plant defense response. UV-2450 analysis suggested...