| Chlorine dioxide is a strong oxidant and is acknowledged as the world's ideal and excellent performance food fresh-keeper and bactericide. Chlorine dioxide has very strong effect of oxidation, which can inactivate microorganism effectively and is a highly efficient and safe antiseptic. It features preventing the formation of ethylene from Methionine and destroying the formed ethylene, thereby providing advantage in the food fresh–keeping. In 1996, GB-2760 in China ranked stable chlorine dioxide as food additive and confined it in fruit and vegetable's fresh keeping and fish processing. In 2004, U.S. FDA authorized chlorine dioxide as antimicrobial for fruits and vegetables. This paper planed to control microbiology on the surfaces of fruits by apple, Kyoto grape, Saccharomyces cerevisiae, E.coli, Rhizopus stolonifer, Botrytis cinerea as as experimental materials. The mechanism and the influenced factors were analyzed. The aim of this study was to provide the fundamental theories for the application of chlorine dioxide in fresh keeping and food safety. The main conclusions were as follows:1 Escherichia coli 8099 inoculated on the kyoho grape surfaces were treated by chlorine dioxide solution. The treatment time, concentration of chlorine dioxide solution and population of the inoculated E.coli 8099 which influence the inactivation efficacy of chlorine dioxide were studied by single-factor and orthogonal rotary design method, respectively. The mathematical model was established. The concentration of chlorine dioxide was the most important factor of influncing inactivation efficacy of chlorine dioxide, and the treatment time was the second, the population of the inoculated E.coli 8099 was the third. Both the concentration of chlorine dioxide and the treatment time significantly improved the inactivation efficacy (P<0.01).2 Saccharomyces cerevisiae 1450 cells inoculated on the kyoho grape surfaces were treated by chlorine dioxide solution. The treatment time, concentration of chlorine dioxide and the population of the inoculated... |