Primary Study About The Antiviral Properties Of Antimicrobial Peptides From Northeast Forest Frogs

The skin antimicrobial peptides(AMPs) are the barrier for amphibians to defense invading of microorganism. They have many families and lots of functions, such as anti bacteria, anti fugin and anti tumor. Recently, it reported that AMPs have inactivate properties to herpes virus and HIV-1, and simultaneity no harm for host cells. Nowadays the spread of virus diseases depress the globle economy, to find safe, effective antiviral medicine became the focus. For the unique characters of amphibian skin peptides, it is hoped to be the important way of exploring new antibiotic.In this paper, we isolate and purified AMPs from northeast forest frogs(Rana dybowskii), choose the SAH hydrolase as the antiviral filtration model to determine the broad-spectrum antiviral properties, then choose the zoonosis virus—H9N2 avian influenza virus to investigate its inactive properties to enveloped virus.Result: (1) We extract and purified SAH hydrolas from bovine liver, the specific activity of the extract SAH hydrolase is 1.23U/mg, it can catalyse 30.7% Hcy to SAH within 1 minute;(2) When put the skin peptide of Northeast forest frog into the reaction system, there is 44.7% Hcy change into SAH within 1 minute, It indicate that the skin peptide of Northeast forest frogs regress the activity of SAH hydrolase, which indicate that the skin activity peptide has broad- spectrum activity;(3) The maximum nontoxic concentration is 150mg/ml. there are comparison group, infection group, killing group, and therapy group and prevent group in this experiment. Compared with infection group, the titer of therapy group and prevent group, the killing group differ significant(P<0.01), the HA of killing guoup reduced from (10±0.82) to (3 ±0. 82), the HA of prevent group reduce by (5. 5 ± 0. 50) , HA of therapy group reduce to (5.3 ± 0.58) , compared with therapy group and prevent group, the killing group shows distinct effect on the avian influenza virus H9N2.Conclusion: (1)We establish antiviral filtration model in vitro— SAH hydrolase model, and examine its activity from the synthesizes direction with ultraviolet spectrophotometer, it is our innovation. This method has high accuracy, short periods and can be easy operated, it is adapt to be spread out;(2) the skin peptide of Northeast forest frogs regress the activity of SAH hydrolase, which indicate that the skin activity peptide has broad- spectrum activity;(3)the skin AMPs of northeast forest frogs have inactive properties to H9N2 avian influenza virus, indicate that AMPs of northeast forest frogs can inactive evoloped virus.