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Inheritance Analysis Of Resistance To Cotton Worm (Prodenia Litura, Fabricius), QTL Mapping And Cloning Of Related Genes In Soybean

Posted on:2006-07-14Degree:MasterType:Thesis
Country:ChinaCandidate:H LiuFull Text:PDF
GTID:2133360185965875Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
One objective of this study was to determine the inheritances of resistance to cotton worm (Prodenia litura, Fabric ius) in the net room. The two soybean Recombinant Inbred Lines (RIL)(Kefeng No.1×Nannong1138-2 and Wan82-178×Tongshanbopihuangdoujia) were used as experimental materials. The leaves from each line were collected. Cotton worm larvae were reared on individual lines of the RIL populations, the larvae and pupae weightsof cotton worm were examined as index of resistance. The result indicated that both larvae and pupae weights were controlled by two major genes plus polygenes. As far as the first RIL populations were concerned, analysis of mean weight of surviving larvae and pupae indicated that two major genes and polygenes controlled the inheritances. The heritability values of major genes were about 66.48% and 66.96%, and the polygene heritability values were about 19.90% and 24.84%. Analysis of mean larvae weight demonstrated the similar result, the heritability values of major genes of the second RIL populations were higher than the first one, and reached to 89.85%, but the heritability values of polygenes were only 4.23%.Based on the linkage maps constructed with SSR markers of the two RIL populations and the QTL Cartgrapher software, another objective of this study was to determine quantitative trait loci (QTLs) associated with the soybean insect resistance. To the kefeng No.1×Nannongl 138-2 populations, four QTLs related to this trait were mapped on linkage groups G20-O, G7-Dla+Q, G10-E and G17-L, and the position were between 0.01cM to 31.91cM, they explained from 5.60% to 11.74% of the total variation of the larvae and pupae weight. As far as the Wan82-178xTongshanbopihuangdoujia RIL populations were concerned, two QTLs related to this trait were mapped on linkage group wt-11 and wt-12, and the position were 5.51cM and 11.51cM, which explained 17.22% and 8.60% of the total variation of the larvae weight, respectively.
Keywords/Search Tags:Soybean(Glycine max (L.)Merrill), Cotton worm (Prodenia litura, Fabricius), Insect resistance gene, Quantitative trait loci (QTLs)
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