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Studies On Insect Virus Microbe Multiple Insecticide Applying Technique And Production

Posted on:2006-06-09Degree:MasterType:Thesis
Country:ChinaCandidate:Y W SunFull Text:PDF
GTID:2133360185965884Subject:AE01 planting
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The productivity and quality of vegetables were affected by the serious damage of many insect pests including Spodoptera litura Fabricius, Spodoptera exigua (Hubner) and Plutella xylostella Linnaeus. In recent years, the problem of "3R" and the pollution became more and more serious with the increasing usage of chemical insecticides. Nuclear polyhedrosis viruses (NPVs) have received considerable attention due of their efficacy, specificity, production of secondary inoculum, and the lack of non-target effects, and theyprovide attractive alternatives to broad-spectrum insecticides and have the potential to form components of integrated pest management (IPM) programmes. In this paper, the study focused on the co-effect between insect virus and Bacillus thuringiensis and their applying technique, at the same time, it also involved the comparison of methods for NPV collection and host insect for virus propagation. All these will provide the guidance for insect virus production.The co-effect between NPV and Bt against 2nd instar Plutella xylostella was tested by leaf-dropping method. It was found that the toxicity regression equation of the best ratio was y=4.8036+0.3883x, and the lethal concentration of 50% individuals (LC50) was 3.16 PIB/ml, the CTC between NPV and Bt was 500.55, which indicated that there was significantly synergistic effect between NPV and Bt.Field trails of the multiple insect virus pesticide was carried out in suburb of Nanjing city. The results showed that, after sprayed for 7d, the efficacy of 300 times dilution of the multiple pesticide against Plutella xylostella and Spodoptera exigua were 76.35% and 78.95%, respectively. Although the persistence of this pesticide was fine, but its killing speed was slow. Thus, the insect virus microbe pesticides must combine with chemical pesticides when insect pests break out.
Keywords/Search Tags:Insect viruses, Spodoptera litura Fabricius, Spodoptera exigua (Hübner), Plutella xylostella Linnaeus, CTC, field efficacy, method for NPV collection, host insect
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