Experimental Study On The Apoptosis Of Human Tongue Squamous Cell Carcinoma Cell Line CAL - 27 Induced By Lactobacillus A - 2 Metabolites

Objective:To discuss the effect of Lactobacillus sp A-2metabolites on proliferation of CAL-27cellsand apoptosis in CAL-27cells.Methods:Lactobacillus sp metabolites1and2(LSPM1and LSPM2) were obtained by culturingLactobacillus sp A-2in rescongtitution whey medium and rescongtitution whey-inulin medium;the proliferation of the CAL-27cells of LSPM1and LSPM2was assayed by Methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed underfluorescence microscopy by Acridine orange (A0) fluorescent staining; flow cytometry method(FCM) and agarose gel electrophoresis were used to detect the apoptosis situation of CAL-27cells were treated with LSPM1and LSPM2.Results:In LSPM1and LSPM2in3.0mg/mL~48mg/mL range, with the increase of metabolites,the inhibitory effect of CAL-27cells gradually strengthened; under the fluorescence microscope,apoptotic cells were irregular, cell shrinkage, condensation crescent-shaped or horseshoe,malapposition; CAL-27treated by LSPM1and LSPM2apoptosis rate were higher than inblank control group; agarose gel electrophoresis showed the typical DNA ladder.Conclusion:Lactobacillus sp A-2metabolite can inhibit the growth of human tongue squamous cellcarcinoma CAL-27cells and induce CAL-27cell apoptosis in vitro。...