IPF Related Parameters And Its Role In The Diagnosis Of Thrombocytopenic Diseases

Aim:Identify the immature platelet fraction (IPF) of healthy adults in Tianjin and evaluate its value in diagnosis in thrombocytopenia.Methods:The absolute value of IPF(#), the percent of IPF(%), the percent of high-fluorescence IPF(H-IPF)(%) and PLT were detected in peripheral blood from2600healthy adults,23ITP patients,29aplastic anemia (AA) patients,10AML-M3patients and24aplastic anaemia in complete remission (AA-CR) patients by using XE-5000automated hematology analyzer. Furthermore, we investigated the differences between different groups and analyzed the diagnosis value of these parameters.Results:The IPF(#), IPF(%), H-IPF(%) and PLT was0.7-5.6×109/L,0.3-2.8%,0-0.8%and143-333×109/L respectively. Compared with healthy control, the IPF(#), IPF(%) and H-IPF(%) in idiopathic thrombocytopenic purpura (ITP) and AML-M3patients significantly increased (P<0.05), while PLT decreased significantly (P<0.05). The IPF(#) and PLT in AA patients significantly decreased(P<0.05), while the IPF(%) was significantly increased compared to healthy control(P<0.05). The IPF(%) and PLT in AA-CR patients significantly decreased compared with healthy control. The diagnosis sensitivity, specificity and maximum youden’s index of IPF(%) were90%,95.7%and85.7%respectively in ITP patients. The diagnosis sensitivity, specificity and maximum youden’s index of IPF(#) were90.0%,100%and90%respectively in AA patients. The diagnosis sensitivity, specificity and maximum youden’s index of H-IPF(%) were98.3%,69.7%and77.9%respectively in ITP patients. The diagnosis sensitivity, specificity and maximum youden’s index of H-IPF(%) were82.5%,90.0%and72.5%respectively in AML-M3patients.Conclusions:This study establishes the reference ranges of IPF(#), IPF(%) and H-IPF(%) in healthy adults in Tianjin, and proves that IPF(%) and IPF(#) have the important value in the diagnosis of ITP and AA respectively and H-IPF(%) has the important value in the diagnosis of both ITP and AML-M3. Aim:Investigate the reference ranges of parameters of blood cells in venous blood in healthy adults.Methods:We performed complete blood count in2600healthy adults(male:1300, female:1300, age:18-60) by using XE-5000automated hematology analyzer.Results:The NEUT-X value was1240-1362(10Λ(-l)ch) and1239-1359(10Λ(-l)ch) for male and female respectively; the RET-Y was1690-1872(10Λ(-l)ch) and1603-1844(10Λ(-l)ch); FRC%was0-30(10Λ(-2)%) and0-98(10Λ(-2)%) for male and female respectively; FRC(#) was0-145(10Λ2/μL) and0-446(10Λ2/μL) for male and female respectively; the RPI was5-21(10Λ(-l)ch) and3-14(10Λ(-l)ch) for male and female respectively; RBC-He was281-33l(10Λ(-l)pg) and263-324(10Λ(-l)pg) for male and female respectively; the HFLC#was0-2(10/μL) and0-2(10/μL) for male and female respectively; the HFLC%was0-3(10Λ(-l)%) and0-5(10Λ(-l)%) for male and female respectively; the PLT-X was93-153(10Λ(-l)ch) and93-157(10Λ(-l)ch) for male and female respectively; the IPF#was8-56(10Λ2/μL) and7-57(10Λ2/μL) for male and female respectively; H-IPF%was0-8(10Λ(-l)%) and0-8(10Λ(-l)%) for male and female respectively; the Area#was0-2(/μl) and0-l(/μl) for male and female respectively; the Area%was0-4(10Λ(-2)%) and0-2(10Λ(-2)%) for male and female respectively; the difference between RET-He and RBC-He(D-He) was18-42(10Λ(-l)pg) and6-41(10Λ(-l)pg) for male and female respectively; the RBC-Y was1602-1769(10Λ(-l)ch) and1535-1747(10Λ(-l)ch) for male and female respectively; the LScRBC%was l-5(10Λ(-l)%) and2-20(10Λ(-l)%) for male and female respectively; the HScRBC%was5-ll(10Λ(-l)%) and3-10(10Λ(-l)%) for male and female respectively; the MicroR%was4-24(10Λ(-l)%) and4-42(10Λ(-l)%) for male and female respectively; the MacroR%was60-120(10Λ(-l)%and50-100(10Λ(-l)%) for male and female respectively; the IRF-Y was1542-2077(10Λ(-l)ch) and0-2027(10Λ(-l)ch) for male and female respectively.Conclusion:There are significant differences between these20parameters of blood cells and this study provides us the normal ranges of these20parameters which are important references for the diagnosis of diseases in clinic.