Molecular Characterization Of Isolated Myosinophilic Acid - Resistant Mycobacterium Tuberculosis In Beijing Area

Tuberculosis remains a great threat to global health especially to developing countries. China is a TB-high burden country which the prevalence is second to India’s. Meanwhile, pulmonary TB belonged to category B notifiable disease in China. According to annual report published by National Health and Family Planning Commission of the People’s Republic of China, there were951,508new cases of tuberculosis in2012. Mycobacterium tuberculosis is the leading cause of tuberculosis, which belongs to Mycobacterium genus. In addition, other species of Mycobacterium genus named as Non-tuberculosis mycobacterium (NTM), is also involved in human tuberculosis. Recent years, the prevelance of MDR-TB (resistant to at least rifampicin and isoniazide) and XDR-TB (MDR-TB with additional resistance to any fluoroquinolone and to at least one of three injectable drugs used for TB treatment: capreomycin, kanamycin, or amikacin) has brought great challenge to prevention and treatment of tuberculosis. Para-aminosalicylic acid is one of the important drugs to treat MDR-TB. The emergence of PAS-resistant strains would lead to failure of TB treatment.205PAS resistant isolates collected from patients in Beijing, China. The molecular characteristics were determined by performing16S rDNA species identification, spoligotyping and detecting mutations in drug resistance associated genes (folC, thyA, ribD). The result showed that the majority of PAS-resistant isolates were Mycobacterium tuberculosis, in which90%belonged to Beijing family. There were64%of PAS-resistant M. tuberculosis strains detected mutations in these genes. The study revealed mutations of the critical enzyme coded genes in M.tuberculosis folate metabolic pathway were responsible for the development of PAS-resistance in clinical isolates. Besides, it was noticed that several NTM species were involved in the high level PAS-resistance in clinical isolates. These results would help us understanding the mechanism of drug resistance and provide valiable information for establishing the detecting method of molecular biology diagnosis in drug resistance. Neisseria meningitidis is the causative agent of meningitidis and septicemia in children and young adults worldwide. Neisseria meningococcus is gram-negative bacterium. Human are the only host for meningococcus. In China, serogroup A Neisseria meningitidis is the prevalent strain and strain ST-5complexes was as the predominant lineage and had ever been prevalent in China, the ST-7then replaced ST-5clone as the dominant cause of the disease. Determination of the genome sequence of the prevalent genotype strain would help us understand the genetic background of the evolutionary and epidemiological properties. Here, we sequenced the complete genome of Neisseria meningitidis NMA510612. Strain NMA510612was isolated from a bacterial meningitis patient of Sichuan Province in China in2006.The genome was sequenced using454pyrosequencing (Roche) according to the manufacture’s recommendations combined with Sanger sequencing. The complete genome was analyzed. The total length of NMA510612was2.1Mbp with an average GC contents51.5%,2461proteins,59tRNA and4rRNA. The NMA510612genome shows the highly collinear with WUE2594. Meanwhile we found polymorphic regions existed in genes encoding type IV pilus proteins and type Ⅰ restriction enzyme of NMA510612genome, it had significance to the next function study.