Study On Preparation And Properties Of New Chitosan Drug Delivery System

The orthopedic diseases mainly include bone defection, osteoarthritis and bone tumor, which will bring serious influence to the life of patients. The current treatment is mainly about filling of bone defection and transplantation, etc. A large number of scholars used preparation with drugs (fat-soluble and water-soluble drugs) to treat different bone diseases. The drugs are Taxol, Icaritin and BMP, which have good curative effect of orthopedic diseases. What is more, all of them are widely applied in different fields. Chitosan which has good biodegradability and biocompatibility get more and more scholars paying their attention on it as a main raw material to create the carriers. In this paper, we used chitosan as the main raw material to create two different kinds of medicine systems. They are PLGA-chitosan one way slow release membrane and injected chitosan semi-fluid drug delivery system. By carrying different solubility of drugs, we tested the effect of treatment of different bone diseases.This study was divided into three parts:1. Comparing with two different kinds of raw materials (gelatin and chitosan) we could make sure that the chitosan can be more suitable inner membrane. We decided to use PLGA which had good film-forming property and slower degradation rate to create the PLGA-chitosan one-way slow controlled release drug delivery system. The BSA as a model drug tested physical characteristics of drug delivery system (degradability, swelling property, surface morphology and release behavior) and biological compatibility.2. Using chitosan made a injected chitosan semi-fluid drug delivery system (ICS). (1) the taxol was model drug and the physical properties of ICS was tested (degradability, swelling, release behavior) and biocompatibility and antitumor efficacy. (2) ICS with icaritin treated cartilage defect. Through the animal experiment to test the effect of cartilage defect repair, studies shew that drug delivery system with icaritin could differentiate mesenchymal stem cells.3. We transfected plasmid p5036-rhBMP-2/2 and p5037-rhBMP-2-2/7 into chinese hamster ovary cells (CHO). Then we got the products of BMP-2 and BMP-2-7. By cell test and physical characteristics test we got the concentration and differentiated function of BMP-2 and BMP-2-7.