Effects Of Tripterine On Expression Of RANKL In Microenvironment Of CIA Mice

objective: to develop the effects of Celastrol on RANKL in bone marrow microenvironment of CIA mousemethods: CIA mouse model was established to observe treatment effect of Cel for CIA and the effect in bone marrow microenvironment. Including:1.Observing inflammation and record the arthritis score of CIA mouse model pathogenesis; to determine the expression of RANKL in T cell\B cell\ mononuclear cells\lymphocyte in bone marrow microenvironment. through CIA mouse model pathogenesis and use tartrate-resistant acid phosphatase(TRAP) staining to observe the differentiation of bone marrow cells to osteoclasts. 2. Recording the arthritis scores of different groups(CIA\CIA+Cel low\middle\high consentration),we observe the treatment effect of Cel; to determine the expression of RANKL in T cell\B cell\ mononuclear cells\lymphocyte in bone marrow microenvironment in different groups; and use tartrate-resistant acid phosphatase(TRAP) staining to observe the differentiation of bone marrow cells to osteoclasts in different groups.results: The arthritis score results showed that comparing the CIA mouse model group, the arthritis score of CIA+Cel low concentration\ CIA+Cel middle concentration\ CIA+Cel high concentration all declined, and the CIA+Cel high concentration group was the lowest. The flow cytometry results demonstrated that the expression of RANKL in T cell\B cell\ lymphocyte of bone marrow of CIA mouse model were all at the top during the development of disease, and then decreased. The ratio of RANKL in plasma cell increased at remission stage during the development of disease. And the RANKL in T cell\B cell\ mononuclear cells\lymphocyte of CIA+Cel different concentration groups all decreased, while the ratio of RANKL in plasma cell increased in CIA+Cel different concentration groups.. Meanwhile, the osteoclast differentiation results showed that the bone marrow of CIA model mouse could differentiate to osteoclast at lower concentration of RANKL, especially in the early stage during the development of disease, while the osteoclast differentiation induced of CIA+Cel different concentration groups bone marrow.conclusion: The expression of RANKL in bone marrow microenvironment of CIA mouse model might change before the arthritis performance. Cel could inhibite inflammation and decrease the ratio of RANKL in T cell\B cell\ mononuclear cells\lymphocyte in CIA mouse bone marrow, increase the ratio of RANKL in plasma cell,which meant that Cel might change the bone microenvironment in CIA mouse model.