Protective Effects Of Hydroxyl Radical On Reducing Acute Hepatic Injury Of Carbon Tetrachloride By

ObjectivesSedum sarmentosum(SS), a Chinese herb was widely used for hepatopathy treatment in clinical practice. Three different acute liver injury murine models were used to study the effect of SS on oxidative stress response in liver and to further explore the target of SS on acute liver injury. This study will provide experimental evidence for its clinical practice.Methods1. Three different acute liver models were used, C57BL/6 mice were administrated by single intraperitoneal injection with CCl4, D-GalN/LPS and single intravenous injection with ConA, respectively. All of the mice were treated with SS by gastrogavage and PBS at the same time. Survival rate of mice, serum aminotransferase level and liver pathology with HE staining were used to evaluate the effect of SS on protecting liver injury.2. Level of XOD, O2-, T-SOD, GSH-Px and MDA in liver homogenate were analyzed to study the influence of SS on oxidative stress response in three acute liver injury models.3. Level of OH·,4-HNE and 8-ISO-PGF2α in liver homogenate were detected to further explore the target of liver protection of SS in acute liver injury which was induced by CCl4.Results1. SS protected the CCl4 induced acute liver injury, increased the survival rate of mice, and decreased serum aminotransferase level, as well as reduced the pathological damage of liver.2. SS reduced oxidative stress injury of liver in the model which was induced by CCl4, and improved capacity to inhibit OH·, as well as decreased MDA production which was the end product of lipid peroxidation.Conclusions1. SS is an effective Chinese herb to attenuate acute liver injury induced by CCl4 in mice.2. SS may protect the CCl4 induced liver injury by inhibiting the production of OH·, relieving lipid peroxidation and decreasing MDA.