Changes Of Long - Chain Non - Coding RNA In Chronic Obstructive Pulmonary Disease

Part1Microarray analysis of long non-coding RNAs in COPD lung tissueBackground:Long noncoding RNAs （lncRNAs） play an important regulatory role in a variety of biological and pathological processes and are implicated in the pathogenesis of many human diseases. In this study, we describe the expression of lncRNAs and mRNAs in lung tissue form three non-smokers, five smokers without chronic obstructive pulmonary disease （COPD） and five smokers with COPD using microarray analysis.Methodology:RNA was extracted from human lung tissue and analysed using an Agilent Human lncRNA+mRNA Array v2.0system. Gene Ontology （GO） and pathway analysis was then performed and mapped genes to the Kyoto Encyclopedia of Genes and Genomes （KEGG） database.Results:39,253distinct IncRNA transcripts were detected in the lung tissues of all subjects. In smokers without COPD87lncRNAs were significantly up-regulated and244down-regulated compared to non-smokers with RNA50010UCSC-9199-1005（fold-change:13.02297） and RNA58351CombinedLit₃16₅50（fold-change:12.609704） the most over-and under-regulated respectively.In contrast, in COPD patients120lncRNAs were overexpressed and43underexpressed compared with smokers without COPD with RNA44121|UCSC-2000-3182（fold-change:8.721127） and RNA43510|UCSC-1260-3754（fold-change:5.527549） being the most over-and under-regulated respectively. GO and pathway analysis indicated that cigarette smoking was associated with activation of metabolic pathways and whereas COPD transcripts were associated with ’hematopoietic cell lineage’, intermediary metabolism and immune system processes. Conclusions/Significance:Our study is the first to determine the genome-wide expression of lncRNAs in lung tissue by microarray analysis. The results show that cigarette smoking and the presence of COPD alters the expression of lncRNAs in lung tissues and that these incRNAs might play a role in modulating pathways implicated in disease onset and progression such as intermediary metabolism and the immune system. Part2The anti-inflammatory effects of lncRNA-ENST00000548657in HBEObjective:To investigate the role of lncRNA-ENST00000548657in the expression of IL-6and IL-8in human bronchial epithelial cell （HBE）.Methods:The HBE were cultured with or without cigarette smoking extract （CSE） stimulation. SiRNA interference was employed to investigate the regulation of IL-6and IL-8by ENST00000548657.Results:CSE could significantly promote the expression of IL-6and IL-8n HBE cells. a The expression of IL-6and IL-8were decreased after siRNA interference in HBE cells. Action of the CSE can partially be antagonized by siRNA interference.Conclusion:lncRNA-ENST00000548657play a role in regulating the expression of inflammatory factors, which may be involved in the pathogenesis of COPD.